食品免疫学小鼠实验有哪些 裸鼠与联合免疫缺陷小鼠有何区别
\u5c0f\u9f20\u548c\u5927\u9f20\u5728\u751f\u7269\u5b66\u4e2d\u7684\u4e3b\u8981\u5e94\u7528\u4ef7\u503c\u6709\u54ea\u4e9b\u9f20\u7c7b\u4e2d\u4e3b\u8981\u5e38\u7528\u5b9e\u9a8c\u54c1\u79cd\u4ecb\u7ecd\u2014\u2014\u5c0f\u9f20----------
\u5c0f\u9f20\uff08mouse\uff09\uff0c\u5b66\u540d\uff1amus musculus,\u5728\u751f\u7269\u5206\u7c7b\u5b66\u4e0a\u5c5e\u810a\u690e\u52a8\u7269\u95e8\u3001\u54fa\u4e73\u52a8\u7269\u7eb2\u3001\u556e\u9f7f\u76ee\u3001\u9f20\u79d1\u3001\u9f37\u9f20\u5c5e\u3001\u5c0f\u5bb6\u9f20\u79cd\u3002
\u5c0f\u9f20\u54c1\u79cd\u4e4b\u4e00\uff1aICR\u5c0f\u9f20
\u751f\u6d3b\u4e60\u6027
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\u5e38\u7528\u54c1\u7cfb
\u8fd1\u4ea4\u7cfb\uff08inbred strain\uff09:
BALB/c\u5c0f\u9f20\u5f62\u6210\u4e86\u8bb8\u591a\u4e9a\u7cfb\uff0c\u5982BALB/cAnN, BALB/cJ\uff0cBALB/cCd\u3002BALB/c\u5c0f\u9f20\u57fa\u56e0\u578b\u4e3aAabbcc\u3002\u6bdb\u8272\u4e3a\u767d\u8272\u3002\u5176\u4e73\u817a\u764c\u53d1\u75c5\u7387\u4f4e\uff0c\u4f46\u5bf9\u81f4\u764c\u56e0\u5b50\u654f\u611f\u3002\u4e73\u817a\u80bf\u7624\u53d1\u751f\u7387\u7ea6\u4e3a10\uff05~20\uff05\u3002\u6709\u4e00\u5b9a\u6570\u91cf\u7684\u5375\u5de2\u3001\u80be\u4e0a\u817a\u548c\u80ba\u90e8\u80bf\u7624\u3001\u767d\u8840\u75c5\u7684\u53d1\u751f\u3002\u80ba\u764c\u53d1\u75c5\u7387\u96cc\u602726\uff05\uff0c\u96c4\u602729\uff05\u3002\u767d\u8840\u75c5\u53d1\u75c5\u7387\u96cc\u602712\uff05\uff0c\u96c4\u602710\uff05\u3002\u8840\u538b\u4e0e\u5176\u4ed6\u8fd1\u4ea4\u7cfb\u5c0f\u9f20\u76f8\u6bd4\u4e3a\u6700\u9ad8\uff0c\u6709\u81ea\u53d1\u9ad8\u8840\u538b\u75c7\u3002\u8001\u5e74\u5c0f\u9f20\u5fc3\u810f\u6709\u67d0\u4e9b\u75c5\u53d8\uff0c\u96cc\u96c4\u5c0f\u9f20\u5e38\u6709\u52a8\u8109\u786c\u5316\u3002\u51e0\u4e4e\u5168\u90e820\u6708\u9f84\u7684\u96c4\u6027\u5c0f\u9f20\u5747\u6709\u6dc0\u7c89\u6837\u53d8\u3002\u5bf9\u9f20\u4f24\u5bd2\u6c99\u95e8\u6c0f\u83ccC`5\u654f\u611f\uff0c\u5bf9\u9ebb\u75b9\u75c5\u6bd2\u4e2d\u5ea6\u654f\u611f\uff0c\u6613\u60a3\u6162\u6027\u80ba\u708e\uff0c\u5bf9\u653e\u5c04\u7ebf\u6781\u5ea6\u654f\u611f\u3002\u5bcc\u4e8e\u7f51\u72b6\u5185\u76ae\u7ec6\u80de\u7684\u5668\u5b98\uff08\u5982\u809d\u3001\u813e\uff09\u4e0e\u4f53\u91cd\u76f8\u6bd4\uff0c\u6240\u5360\u6bd4\u503c\u5f88\u5927\u3002\u5e38\u7528\u4e8e\u5355\u514b\u9686\u6297\u4f53\u548c\u514d\u75ab\u5b66\u7814\u7a76\u3002 BALB/c\u5c0f\u9f20\u751f\u4ea7\u6027\u80fd\u597d\uff0c\u7e41\u6b96\u671f\u957f\uff0c\u4e00\u822c\u65e0\u76f8\u4e92\u4fb5\u88ad\u4e60\u6027\uff0c\u6bd4\u8f83\u5bb9\u6613\u7fa4\u517b\u3002\u5e73\u5747\u5bff\u547d\uff1a\u6709\u7684\u8bb0\u8f7d\u96c4\u9f20\u4e3a509\u5929\uff0c\u96cc\u9f20\u4e3a561\u5929\uff1b\u6709\u7684\u8bb0\u8f7d\u96c4\u9f20\u4e3a648\u5929\uff0c\u96cc\u9f20\u4e3a816\u5929\u3002\u5e73\u5747\u4f53\u91cd252\u65e5\u9f84\u96c4\u9f20\u4e3a30 g\uff0c\u96cc\u9f20\u4e3a28g\u3002
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1\u5b89\u5168\u6027\u548c\u6bd2\u6027\u8bd5\u9a8c \u5e38\u9009\u7528\u5c0f\u9f20\u8fdb\u884c\u98df\u54c1\u3001\u5316\u5986\u54c1\u3001\u836f\u7269\u5316\u5de5\u4ea7\u54c1\u7b49\u7684\u5b89\u5168\u65b0\u5b9e\u9a8c\uff0c\u6025\u6027\u3001\u4e9a\u6025\u6027\u3001\u6162\u6027\u3001\u6bd2\u6027\u8bd5\u9a8c\uff0c\u8fd8\u53ef\u505a\u81f4\u7578\u3001\u81f4\u764c\u81f4\u7a81\u53d8\u8bd5\u9a8c\uff0c\u534a\u6570\u81f4\u6b7b\u91cf\u6d4b\u5b9a\u7b49\u3002
2 \u751f\u7269\u6548\u5e94\u6d4b\u5b9a\u548c\u836f\u7269\u6548\u4ef7\u6bd4\u8f83 \u5e7f\u6cdb\u7528\u4e8e\u8840\u6e05\u3001\u75ab\u82d7\u7b49\u751f\u7269\u5236\u54c1\u7684\u9274\u5b9a\uff0c\u8fdb\u884c\u751f\u7269\u6548\u5e94\u5b9e\u9a8c\u548c\u5404\u79cd\u836f\u7269\u6548\u4ef7\u6d4b\u5b9a\u3002
3 \u836f\u7269\u7684\u7b5b\u9009 \u7b5b\u9009\u8bd5\u9a8c\u591a\u534a\u4ece\u5c0f\u9f20\u505a\u8d77\uff0c\u7b5b\u9009\u5404\u79cd\u836f\u7269\u5bf9\u75be\u75c5\u6709\u65e0\u9632\u6b62\u4f5c\u7528\uff0c\u901a\u8fc7\u7b5b\u9009\u83b7\u5f97\u6bcf\u4e2a\u836f\u7269\u7684\u7597\u6548\u6548\u679c\u540e\uff0c\u518d\u7528\u5176\u4ed6\u52a8\u7269\u8fdb\u4e00\u6b65\u80af\u5b9a\u3002
4\u5fae\u751f\u7269\u5bc4\u751f\u866b\u75c5\u5b66\u7814\u7a76 \u5c0f\u9f20\u5bf9\u591a\u79cd\u75c5\u539f\u4f53\u6709\u654f\u611f\u6027\uff0c\u5c24\u5176\u662f\u5728\u75c5\u6bd2\u5b66\u7814\u7a76\u4e2d\u5e94\u7528\u66f4\u5927\u3002\u9002\u5408\u4e8e\u7814\u7a76\u8840\u5438\u866b\u3001\u759f\u75be\u3001\u9525\u866b\u3001\u6d41\u884c\u611f\u5192\u8111\u708e\u3001\u72c2\u72ac\u75c5\u3001\u810a\u9ad3\u7070\u8d28\u708e\u3001\u6dcb\u5df4\u8109\u7edc\u4ece\u8111\u819c\u708e\u3001\u652f\u539f\u4f53\u3001\u5df4\u6c0f\u6746\u83cc\u548c\u6c99\u95e8\u6c0f\u83cc\u7b49\u3002
5 \u653e\u5c04\u5b66\u7814\u7a76 \u5c0f\u9f20\u5bf9\u653e\u5c04\u7ebf\u7684\u53cd\u5e94\u4e0e\u4eba\u7684\u53cd\u5e94\u6709\u53ef\u6bd4\u6027\uff0c\u53ef\u7528\u6765\u7814\u7a76\u7167\u5c04\u5242\u91cf\u3001\u8f90\u5c04\u6548\u5e94\u7b49\u3002
6 \u80bf\u7624\u767d\u8840\u75c5\u7814\u7a76 \u5c0f\u9f20\u80bf\u7624\u53d1\u75c5\u7387\u9ad8\uff0c\u8fd1\u4ea4\u7cfb\u7ec4\u7ec7\u76f8\u5bb9\u6027\u597d\uff0c\u80bf\u7624\u79fb\u690d\u8f83\u6613\u751f\u957f\uff0c\u56e0\u6b64\u5e94\u7528\u5e7f\u6cdb\u3002\u53ef\u7528\u5c0f\u9f20\u81ea\u53d1\u6027\u80bf\u7624\u7b5b\u9009\u6297\u80bf\u7624\u836f\u7269\u3002\u53ef\u8bf1\u53d1\u5404\u79cd\u80bf\u7624\uff0c\u505a\u6210\u80bf\u7624\u6a21\u578b\uff0c\u8fdb\u884c\u80bf\u7624\u75c5\u56e0\u5b66\u53d1\u75c5\u5b66\u7814\u7a76\u3002\u8fd1\u4ea4\u7cfb\u5c0f\u9f20\u6709\u4e9b\u5c5e\u4e8e\u9ad8\u764c\u7cfb\uff0c\u6709\u4e9b\u5c5e\u4e8e\u4f4e\u764c\u7cfb\uff0c\u5bf9\u7814\u7a76\u80bf\u7624\u53d1\u751f\uff0c\u6bd4\u8f83\u65b9\u4fbf\u800c\u6709\u5229\u3002
7 \u8ba1\u5212\u751f\u80b2\u7814\u7a76 \u5c0f\u9f20\u6709\u89c4\u5f8b\u7684\u53d1\u60c5\u5468\u671f\u3001\u6392\u5375\uff0c\u598a\u9648\u6709\u660e\u663e\u6307\u6807\uff0c\u6613\u4e8e\u68c0\u6d4b\uff0c\u4ef7\u683c\u4fbf\u5b9c\uff0c\u5e38\u7528\u6765\u505a\u6297\u751f\u80b2\u3001\u6297\u7740\u5e8a\u3001\u6297\u65e9\u5b55\u3001\u6297\u6392\u5375\u5b9e\u9a8c\uff0c\u5f88\u9002\u5b9c\u8fdb\u884c\u907f\u5b55\u836f\u7814\u7a76\u3002
8 \u9547\u54b3\u836f\u7814\u7a76 \u5c0f\u9f20\u53c8\u54b3\u55fd\u53cd\u5e94\uff0c\u53ef\u5229\u7528\u8fd9\u4e00\u7279\u70b9\u7814\u7a76\u9547\u54b3\u836f\u7269\uff0c\u6210\u4e3a\u5fc5\u9009\u5b9e\u9a8c\u52a8\u7269\u3002
9 \u9057\u4f20\u6027\u75be\u75c5\u7814\u7a76 \u5c0f\u9f20\u6709\u591a\u79cd\u54c1\u7cfb\uff0c\u6709\u4e9b\u6709\u81ea\u53d1\u6027\u9057\u4f20\u75c5\uff0c\u5982\u5c0f\u9f20\u9ed1\u8272\u7d20\u75c5\u767d\u5316\u75c5\u3001\u5bb6\u65cf\u6027\u80a5\u80d6\u3001\u9057\u4f20\u6027\u8d2b\u8840\u7b49\u3002\u4e0e\u4eba\u53d1\u75c5\u76f8\u4f3c\uff0c\u53ef\u88ab\u7528\u4f5c\u4eba\u7c7b\u9057\u4f20\u6027\u75be\u75c5\u7684\u52a8\u7269\u6a21\u578b\u3002
10 \u514d\u75ab\u5b66\u7814\u7a76 \u5404\u79cd\u514d\u75ab\u7f3a\u9677\u5c0f\u9f20\uff0c\u5982\u7eaf\u7cfb\u65b0\u897f\u5170\u9ed1\u8272\u5c0f\u9f20\uff08NZB\uff09\u6709\u81ea\u8eab\u514d\u75ab\u6027\u6eb6\u8840\u6027\u8d2b\u8840\uff0cAKR/N\u54c1\u7cfb\u5c0f\u9f20\u53c8\u8865\u4f53C5\u7f3a\u635f\uff0cCBA/N\u5c0f\u9f20\u65e0B\u7ec6\u80de\u7684\u514d\u75ab\u7f3a\u9677\u7b49\uff0c\u90fd\u662f\u7814\u7a76\u514d\u75ab\u673a\u7406\u548c\u514d\u75ab\u7f3a\u9677\u75c5\u7684\u826f\u597d\u5b9e\u9a8c\u52a8\u7269\u3002
11 \u8001\u5e74\u5b66\u7814\u7a76 \u5c0f\u9f20\u5bff\u547d\u77ed\uff0c\u4f20\u4ee3\u65f6\u95f4\u77ed\uff0c\u4f7f\u4ed6\u4eec\u5728\u8001\u5e74\u5b66\u7814\u7a76\u4e2d\u6781\u4e3a\u6709\u7528\u3002\u5f88\u591a\u6297\u8870\u8001\u836f\u7269\u7684\u7814\u7a76\u53ef\u5728\u5c0f\u9f20\u4e0a\u8fdb\u884c\u3002
\u9f20\u79d1\u5e38\u7528\u5b9e\u9a8c\u54c1\u79cd\u4ecb\u7ecd\u2014\u2014\u5927\u9f20----------
\u5927\u9f20\uff08RAT\uff09\u5b66\u540dRattus norvegicus,\u5728\u751f\u7269\u5206\u7c7b\u5b66\u4e0a\u5c5e\u810a\u690e\u52a8\u7269\u95e8\u3001\u54fa\u4e73\u52a8\u7269\u7eb2\u3001\u556e\u9f7f\u76ee\u3001\u9f20\u79d1\u3001\u5bb6\u9f20\u5c5e\u3001\u8910\u5bb6\u9f20\u79cd\u3002
\u751f\u6d3b\u4e60\u6027
\u751f\u957f\u53d1\u80b2\uff1a\u65b0\u751f\u5927\u9f20\u4f53\u91cd\u7ea65-6\u9897\uff0c45\u5929\u4f53\u91cd\u53ef\u8fbe180\u514b\u4ee5\u4e0a\uff0c\u6b64\u65f6\u53ef\u4f9b\u8bd5\u9a8c\u7528\u3002\u6210\u5e74\u96c4\u6027\u5927\u9f20\u4f53\u91cd\u53ef\u8fbe300-800\u514b\uff0c\u6b64\u884c\u53ef\u8fbe200-400\u514b\u3002
\u6d3b\u52a8\u89c4\u5f8b\uff1a\u5927\u4e66\u559c\u5543\u54ac\uff0c\u767d\u5929\u5e38\u6324\u5728\u4e00\u8d77\u4f11\u606f\uff0c\u591c\u95f4\u6d3b\u52a8\uff0c\u665a\u4e0a\u6d3b\u52a8\u91cf\u5927\uff0c\u91c7\u98df\u91cf\u826f\u591a\uff0c\u98df\u6027\u5e7f\u6cdb\uff0c\u559c\u8089\u98df\u3002\u5bf9\u5149\u7167\u3001\u566a\u97f3\u654f\u611f\u3002
\u7e41\u6b96\u7279\u6027\uff1a\u5927\u9f202\u6708\u9f84\u6027\u6210\u719f\uff0c\u6027\u5468\u671f4-5\u5929\uff0c\u598a\u5a20\u671f\u4e3a19-21\u5929\uff0c\u54fa\u4e73\u671f\u4e3a21\u5929\uff0c\u6bcf\u53f0\u4ea7\u4ed4\u5e73\u57478\u652f\u3002\u53ef\u6839\u636e\u5f15\u5bfc\u6d82\u7247\u89c2\u5bdf\u6027\u5468\u671f\u4e2d\u5f15\u5bfc\u4e0a\u76ae\u53d8\u5316\uff0c\u5224\u65ad\u6027\u5468\u671f\u4e2d\u5404\u4e2a\u65f6\u671f\u4e2d\u5375\u5de2\u3001\u5b50\u5bab\u4e0e\u5782\u4f53\u6fc0\u7d20\u53d8\u5316\u7684\u72b6\u6001\u3002
\u5e38\u7528\u54c1\u7cfb
SD\u5927\u9f20:
\u751f\u957f\u5feb\uff0c\u7e41\u80b2\u6027\u80fd\u597d\uff0c\u5927\u591a\u7528\u4e8e\u5b89\u5168\u6027\u8bd5\u9a8c\u53ca\u8425\u517b\u4e0e\u751f\u957f\u53d1\u80b2\u6709\u5173\u7684\u7814\u7a76\u3002\u8be5\u54c1\u7cfb\u5bf9\u6027\u6fc0\u7d20\u654f\u611f\uff0c\u5bf9\u547c\u5438\u9053\u75be\u75c5\u6709\u8f83\u5f3a\u7684\u62b5\u6297\u529b\u3002\u5e7f\u6cdb\u7528\u4e8e\u836f\u7406\u3001\u6bd2\u7406\u3001\u836f\u6548\u53caGLP\u5b9e\u9a8c\u3002
\u7e41\u6b96\u6027\u80fd\uff1a\u4ea7\u4ed4\u7387\uff1a92\uff5e95\uff05 \uff1b\u5e73\u5747\u7a9d\u4ea7\u4ed4\u6570\uff1a9.96\uff5e12.07\u53ea \uff1b\u80ce\u95f4\u9694\uff1a28\uff5e52\u5929\uff1b\u79bb\u4e73\u5b58\u6d3b\u7387\uff1a95\uff5e98\uff05\u3002
Wistar\u5927\u9f20\uff1aWistar\u5927\u9f20\u7531\u7f8e\u56fd\u8d39\u57ceWistar\u7814\u7a76\u6240\u80b2\u6210\u3002\u5e38\u7528\u7684\u65e2\u6709\u8fd1\u4ea4\u7cfb\uff0c\u4e5f\u6709\u8fdc\u4ea4\u7fa4\u3002\u5176\u88ab\u6bdb\u5448\u767d\u8272\uff0c\u7279\u5f81\u4e3a\u5934\u90e8\u8f83\u5bbd\u3001\u8033\u6735\u8f83\u957f\u3001\u5c3e\u7684\u957f\u5ea6\u5c0f\u4e8e\u8eab\u957f\u3002Wistar\u5927\u9f20\u6027\u60c5\u6e29\u987a\uff0c\u6027\u5468\u671f\u7a33\u5b9a\uff0c\u65e9\u719f\u591a\u4ea7\uff0c\u5e73\u5747\u6bcf\u7a9d\u4ea7\u81ea10\u53ea\u5de6\u53f3\uff0c\u751f\u957f\u53d1\u80b2\u5feb\uff0c\u4e73\u817a\u764c\u53d1\u75c5\u7387\u5f88\u4f4e\uff0c\u5bf9\u4f20\u67d3\u75c5\u62b5\u6297\u529b\u5f3a\u3002
Fisher 344\u5927\u9f20\uff1a\u7b80\u79f0F344\u5927\u9f20\uff0c1920\u5e74\u7531\u54e5\u4f26\u6bd4\u4e9a\u5927\u5b66\u80bf\u7624\u7814\u7a76\u6240Curtis\u80b2\u6210\u3002\u6211\u56fd\u4ece\u7f8e\u56fd\u56fd\u7acb\u536b\u751f\u9662\u5f15\u8fdb\u3002\u4e66\u8fd1\u4ea4\u7cfb\u5927\u9f20\uff0c\u5176\u88ab\u6bdb\u5448\u767d\u8272\u3002\u5e73\u5747\u5bff\u547d2-3\u5e74\uff0c\u65cb\u8f6c\u8fd0\u52a8\u6027\u4f4e\uff0c\u8840\u6e05\u80f0\u5c9b\u7d20\u542b\u91cf\u4f4e\u3002\u539f\u53d1\u548c\u7ee7\u53d1\u6027\u813e\u7ea2\u7ec6\u80de\u514d\u75ab\u53cd\u5e94\u6027\u4f4e\u3002\u4e73\u817a\u764c\u3001\u8111\u5782\u4f53\u817a\u7624\u3001\u7532\u72b6\u817a\u7624\u3001\u777e\u4e38\u95f4\u8d28\u7ec6\u80de\u7624\u53d1\u75c5\u7387\u9ad8\u3002\u5e7f\u6cdb\u7528\u4e8e\u6bd2\u7406\u5b66\u3001\u80bf\u7624\u5b66\u3001\u751f\u7406\u5b66\u7b49\u9886\u57df\u3002
SHR\u5927\u9f20\uff1a\u53c8\u79f0\u81ea\u53d1\u6027\u9ad8\u8840\u538b\u5927\u9f20\uff0c1963\u5e74\u7531\u65e5\u672c\u7cbe\u90fd\u5927\u5b66\u533b\u5b66\u90e8Okamoto\u4eceWistar\u5927\u9f20\u79cd\u9009\u80b2\u800c\u6210\u3002\u5c5e\u7a81\u53d8\u7cfb\u5927\u9f20\uff0c\u5176\u88ab\u6bdb\u5448\u767d\u8272\u3002SHR\u5927\u9f20\u81ea\u53d1\u6027\u9ad8\u8840\u538b\u53d1\u75c5\u7387\u9ad8\uff0c\u4e14\u65e0\u660e\u663e\u539f\u53d1\u6027\u80be\u810f\u6216\u80be\u4e0a\u817a\u635f\u4f24\uff0c\u5fc3\u8840\u7ba1\u53d1\u75c5\u7387\u9ad8\u3002\u4f46\u5176\u751f\u80b2\u80fd\u529b\uff0c\u5b58\u8d27\u5bff\u547d\u65e0\u660e\u663e\u4e0b\u964d\u3002
ACL\u5927\u9f20\uff1aACL\u5927\u9f20\u7531\u54e5\u4f26\u6bd4\u4e9a\u5927\u5b66\u80bf\u7624\u7814\u7a76\u6240Curtis\u548cDunning\u57f9\u80b2\u3002\u5c5e\u8fd1\u4ea4\u7cfb\u5927\u9f20\u3002\u5176\u88ab\u6bdb\u5448\u9ed1\u8272\uff0c\u8179\u548c\u811a\u5448\u767d\u8272\u3002\u5e73\u5747\u5bff\u547d2-3\u5e74\uff0c\u6613\u53d1\u751f\u5148\u5929\u6027\u7578\u5f62\uff0c\u80bf\u7624\u53d1\u75c5\u7387\u9ad8\u3002\u5176\u4ed4\u9f20\u77ee\u5c0f\uff0c\u7e41\u6b96\u529b\u5dee\uff0c\u80da\u80ce\u6b7b\u4ea1\u7387\u9ad8\u3002
\u5e94\u7528\u9886\u57df
1 \u751f\u7406\u5b66\u7814\u7a76 \u5927\u9f20\u5728\u751f\u7406\u5b66\u7814\u7a76\u4e2d\u4ee5\u591a\u7528\u9014\u884c\u4e3a\u7279\u5f81\u3002\u5927\u9f20\u5782\u4f53\u2014\u80be\u4e0a\u817a\u7cfb\u7edf\u53d1\u8fbe\uff0c\u5782\u4f53\u6458\u9664\u6bd4\u8f83\u5bb9\u6613\u3002\u53ef\u7528\u6765\u8fdb\u884c\u80be\u4e0a\u817a\u3001\u5782\u4f53\u548c\u5375\u5de2\u7b49\u5185\u5206\u6ccc\u7814\u7a76\u3002\u5229\u7528\u5927\u9f20\u5bf9\u65b0\u73af\u5883\u6613\u9002\u5e94\uff0c\u6709\u63a2\u7d22\u6027\uff0c\u6613\u8bad\u7ec3\uff0c\u5bf9\u60e9\u7f5a\u548c\u6697\u793a\u654f\u611f\u7684\u7279\u6027\u8fdb\u884c\u884c\u4e3a\u5b66\u7814\u7a76\u548c\u9ad8\u7ea7\u795e\u7ecf\u6d3b\u52a8\u7684\u7814\u7a76\u3002\u5927\u9f20\u65e0\u80c6\u56ca\uff0c\u4f46\u80c6\u603b\u7ba1\u8f83\u5927\uff0c\u53ef\u7528\u80c6\u603b\u7ba1\u63d2\u7ba1\uff0c\u6536\u96c6\u80c6\u6c41\uff0c\u7814\u7a76\u6d88\u5316\u529f\u80fd\u3002
2 \u8425\u517b\u5b66\u7814\u7a76 \u5927\u9f20\u662f\u7b2c\u4e00\u79cd\u7528\u4e8e\u8425\u517b\u5b66\u7814\u7a76\u7684\u5b9e\u9a8c\u52a8\u7269\uff0c\u4e3a\u4eba\u7c7b\u8425\u517b\u7814\u7a76\u505a\u51fa\u4e86\u7a81\u51fa\u8d21\u732e\uff0c\u7ef4\u751f\u7d20D\u5c31\u662f\u7528\u5927\u9f20\u7814\u7a76\u800c\u53d1\u73b0\u7684\u3002\u7531\u4e8e\u5927\u9f20\u6742\u98df\uff0c\u89e3\u5256\u3001\u751f\u7406\u4e0e\u4eba\u76f8\u4f3c\uff0c\u751f\u957f\u4ee3\u8c22\u5feb\uff0c\u5bf9\u5927\u9f20\u7684\u8425\u517b\u7814\u7a76\u5e7f\u6cdb\u800c\u7ec6\u81f4\uff0c\u8d44\u6599\u6781\u4e3a\u4e30\u5bcc\uff0c\u5e38\u7528\u4e8e\u7ef4\u751f\u7d20\u3001\u86cb\u767d\u8d28\u7f3a\u4e4f\uff0c\u6c28\u57fa\u9178\u548c\u9499\u78f7\u4ee3\u8c22\u7684\u7814\u7a76
3 \u4ee3\u8c22\u6027\u75be\u75c5\u7814\u7a76 \u53ef\u5e94\u7528\u5927\u9f20\u7814\u7a76\u52a8\u8109\u7ca5\u6837\u786c\u5316\uff0c\u6dc0\u7c89\u6837\u53d8\u6027\u3001\u9152\u7cbe\u4e2d\u6bd2\uff0c\u5341\u4e8c\u6307\u80a0\u6e83\u75a1\uff0c\u8425\u517b\u4e0d\u826f\u7b49\u4ee3\u8c22\u75c5
4 \u836f\u7269\u5b66\u7814\u7a76 \u5927\u9f20\u8840\u538b\u548c\u8840\u7ba1\u963b\u529b\u5bf9\u836f\u7269\u53cd\u5e94\u654f\u611f\uff0c\u9002\u5408\u7814\u7a76\u5fc3\u8840\u7ba1\u836f\u7269\u7684\u836f\u7406\u3002\u7814\u7a76\u6bd2\u6241\u8c46\u78b1\u7684\u5347\u538b\u4f5c\u7528\u3002\u6b64\u5916\uff0c\u5927\u9f20\u8db3\u8dbe\u6d6e\u80bf\u6cd5\u662f\u6700\u5e38\u7528\u7684\u7b5b\u9009\u65b9\u6cd5\uff0c\u5927\u9f20\u8e1d\u5173\u8282\u5bf9\u708e\u75c7\u53cd\u5e94\u654f\u611f\uff0c\u7528\u4e8e\u5173\u8282\u708e\u836f\u7269\u7814\u7a76\u3002\u5927\u9f20\u8fd8\u662f\u8fdb\u884c\u836f\u7269\u8bc4\u4ef7\u7684\u4e3b\u8981\u5b9e\u9a8c\u52a8\u7269\u3002
5 \u80bf\u7624\u7814\u7a76 \u5f88\u591a\u80bf\u7624\u53ef\u4ee5\u79fb\u690d\u5230\u5927\u9f20\u4e0a\u8fdb\u884c\u7814\u7a76\uff0c\u5927\u9f20\u6613\u60a3\u809d\u764c\uff0c\u53ef\u4eba\u5de5\u590d\u5236\u5927\u9f20\u809d\u764c\u3001\u98df\u7ba1\u764c\u52a8\u7269\u6a21\u578b\u3002\u53ef\u76ae\u4e0b\u63a5\u79cd\u6dcb\u5df4\u8089\u7624\u3002
6 \u9057\u4f20\u5b66\u7814\u7a76 \u5927\u9f20\u7684\u6bdb\u8272\u53d8\u578b\u5f88\u591a\uff0c\u53ef\u7528\u6765\u7814\u7a76\u6bdb\u8272\u8bb0\u5fc6\u9057\u4f20\uff0c\u9a8c\u8bc1\u5b5f\u5fb7\u5c14\u9057\u4f20\u5b9a\u5f8b\u3002\u7531\u9057\u4f20\u75be\u75c5\u7684\u5927\u9f20\uff0c\u5177\u6709\u4e0e\u4eba\u76f8\u4f3c\u7684\u8868\u73b0\u3002\u8111\u79ef\u6c34\u3001\u542c\u89c9\u969c\u788d\u3001\u8033\u804b\u3001\u767d\u5185\u969c\u3001\u5782\u4f53\u77ee\u5c0f\u75c7\u3001\u65e0\u7259\u3001\u65e0\u80c6\u6c41\u3001\u4e18\u8111\u4e0b\u90e8\u5c3f\u5d29\u75c7\u3001\u80a5\u80d6\u4e0e\u9ad8\u8840\u538b\u7b49\u90fd\u662f\u9057\u4f20\u75be\u75c5\u7684\u826f\u597d\u52a8\u7269\u6a21\u578b\uff0c\u8fd8\u53ef\u5236\u6210\u76f8\u4f3c\u4e8e\u4eba\u7684\u5b9e\u9a8c\u8bf1\u53d1\u6027\u9057\u4f20\u7f3a\u9677\u75be\u75c5\u52a8\u7269\u6a21\u578b\u3002
7 \u4f20\u67d3\u75c5\u7814\u7a76 \u7528\u4e8e\u7814\u7a76\u526f\u4f24\u5bd2\u3001\u6d41\u611f\u3001\u538c\u6c27\u83cc\u3001\u5047\u7ed3\u6838\u3001\u9709\u578b\u4f53\u3001\u5df4\u6c0f\u6746\u83cc\u3001\u8461\u8404\u7403\u83cc\u3001\u5ff5\u73e0\u72b6\u94fe\u6746\u83cc\u3001\u9ec4\u66f2\u9709\u83cc\u548c\u70df\u66f2\u9709\u83cc\u7b49\u5fae\u751f\u7269\u53ca\u5176\u5f15\u8d77\u7684\u4f20\u67d3\u75c5\u3002
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e0b\u90e8\u5c3f\u5d29\u75c7\u3001\u80a5\u80d6\u4e0e\u9ad8\u8840\u538b\u7b49\u90fd\u662f\u9057\u4f20\u75be\u75c5\u7684\u826f\u597d\u52a8\u7269\u6a21\u578b\uff0c\u8fd8\u53ef\u5236\u6210\u76f8\u4f3c\u4e8e\u4eba\u7684\u5b9e\u9a8c\u8bf1\u53d1\u6027\u9057\u4f20\u7f3a\u9677\u75be\u75c5\u52a8\u7269\u6a21\u578b\u3002\u3000\u30007\u4f20\u67d3\u75c5\u7814\u7a76\u7528\u4e8e\u7814\u7a76\u526f\u4f24\u5bd2\u3001\u6d41\u611f\u3001\u538c\u6c27\u83cc\u3001\u5047\u7ed3\u6838\u3001\u9709\u578b\u4f53\u3001\u5df4\u6c0f\u6746\u83cc\u3001\u8461\u8404\u7403\u83cc\u3001\u5ff5\u73e0\u72b6\u94fe\u6746\u83cc\u3001\u9ec4\u66f2\u9709\u83cc\u548c\u70df\u66f2\u9709\u83cc\u7b49\u5fae\u751f\u7269\u53ca\u5176\u5f15\u8d77\u7684\u4f20\u67d3\u75c5\u3002\u3000\u30008\u67d0\u4e9b\u75be\u75c5\u7814\u7a76\u5982\u652f\u6c14\u7ba1\u80ba\u708e\u3001\u591a\u53d1\u6027\u5173\u8282\u708e\u3001\u5316\u8113\u6027\u6dcb\u5df4\u817a\u708e\u3001\u4e2d\u8033\u75be\u75c5\u548c\u5185\u8033\u708e\u3002\u5927\u9f20\u809d\u5207\u966460%\u81f370%\uff0c\u4ecd\u6709\u518d\u751f\u80fd\u529b\uff0c\u53ef\u7528\u4e8e\u809d\u5916\u79d1\u5b9e\u9a8c\u3002\u3000\u30009\u7259\u79d1\u5b66\u7814\u7a76\u7528\u53d8\u5f02\u94fe\u7403\u83cc\u63a5\u79cd\u5927\u9f20\u53e3\u8154\uff0c\u7136\u540e\u5582\u7ed9\u542b\u8517\u7cd6\u98df\u7269\uff0c\u5927\u9f20\u7259\u9f7f\u4e0a\u7684\u786e\u7405\u8d28\u86c0\u635f\u5728\u5b8f\u89c2\u548c\u5fae\u89c2\u4e0a\u540c\u4eba\u7684\u86c0\u9f7f\u76f8\u4f3c\uff0c\u53ef\u7528\u6765\u7814\u7a76\u9f8b\u9f7f\u3002\u3000\u300010\u5927\u9f20\u8fd8\u53ef\u7528\u4e8e\u8ba1\u5212\u751f\u80b2\u7684\u7578\u80ce\u5b66\u3001\u907f\u5b55\u836f\u7814\u7a76\u548c\u653e\u5c04\u5b66\u7814\u7a76\u3002\u3000\u3000
用可溶性抗原致敏.准备等体积 PBS 含 25～100 μg 抗原和弗氏完全佐剂的乳化液（200～400 μl/鼠）。用双头带塞的连接器连接两个注射器，一个注射器装抗原，另一个装佐剂。来回推压注射器，使得其内容物从一侧到另一侧注射器，推压 5～10 min 直到得到稳定的乳化液。用 22－G 的针给小鼠腹腔注射（每一个抗原 3～5 只小鼠） 周后，用等体积的含 10～50 μg 抗原的 PBS 和不完全弗氏佐剂配制成（200～400 μl）的乳化液再次腹腔注射。第二次免疫后 7 天，用无菌剪刀或剃毛刀片切掉小鼠尾巴 0.5 cm 采血。收集 100～200 μl 血装于 1.5 ml 的微量离心管。将小鼠放在产热灯下 30～60 s 保暖或用拇指和食指挤压尾巴可以方便采血。血块形成后，用木制涂棒把血块从管和表面移走，不要弄破血块。移走血块后，用 200μl 的移液器把血清装入另一微量离心管。经 ELISA 确定血清抗体滴度。如有需要，用免疫印迹，进一步对抗体定如果滴度太低不能用于融合（≥1/1000），每两周给小鼠加强免疫直至达到足够的应答。如果采血超过 3 次，用剃毛刀切开一侧尾静脉采血。当抗体滴度足够高时（≥1/1000），融合前 3 天，但要在前一次免疫的 2 周后，用含 10～50 μg 抗原的 PBS 溶液腹腔注射（200～400μl）或经皮下尾静脉（50～100μl）注射加强免疫。
在强化免疫的 3 天后进行细胞融合。
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