稀释公式怎么算,取0.5ml原液+4.5ml水,这是稀释多少倍, 我现在做青霉素皮试液, 青霉素皮试液配制方法怎么计算啊???
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\u7528\u6240\u9700\u914d\u7f6e\u4f53\u79ef\u4e58\u4e8e\u9700\u914d\u7f6e\u7684\u6d53\u5ea6\u9664\u4e8e\u5df2\u77e5\u6d53\u5ea6\u65e2\u5f97\uff0c\u598280\u4e07\u5355\u4f4d\u9752\u9709\u7d20\u7a00\u91ca\u621030\u4e07\u5355\u4f4d\uff1a\u5c0680\u4e07\u5355\u4f4d\u9752\u9709\u7d20\u52a0\u751f\u7406\u76d0\u6c34\u6eb6\u89e3\uff0c\u52a02ML\u6eb6\u89e3\uff0c\u5c3180/2=40\u4e07\u5355\u4f4d/ML,\u7136\u540e\u8981\u914d5ML30\u4e07\uff0c\u90a3\u5c31\u662f\u53d65*30/40=3.75ML40\u4e07\u5355\u4f4d\u6eb6\u6db2\u52a0\u7a00\u91ca\u6db2\u7a00\u91ca\u81f35ML\u65e2\u5f97\uff0c\u5176\u4ed6\u76f8\u540c\u3002
\u6269\u5c55\u8d44\u6599\uff1a
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\u53c2\u8003\u8d44\u6599\u6765\u6e90\uff1a\u767e\u5ea6\u767e\u79d1-\u9752\u9709\u7d20
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\u6211\u662f\u8fd9\u6837\u505a\u7684\u5f88\u5b9e\u7528\uff0c\u53ea\u8981\u4f1a\u4e86\u8fd9\u4e2a\uff0c\u4efb\u4f55\u4e00\u652f\u9752\u9709\u7d20\u90fd\u53ef\u4ee5\u4efb\u610f\u914d\u3002\u5177\u4f53\u4e3a\u4ec0\u4e48\u4f60\u53ef\u4ee5\u81ea\u5df1\u7406\u89e3\u3002
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500\ufe55200\u4e07\ufe66500\ufe552000000\ufe661\ufe554000\ufe661\uff0f4000\uff1d1\uff0f40\u00d71\uff0f10\u00d71\uff0f10\uff0c\u6211\u4eec\u5c31\u6309\u7b49\u5f0f\u6700\u540e\u8fb9\u7684\u914d\u5c31\u884c\uff0c\u6211\u4eec\u75285\u6beb\u5347\u9488\u7ba1\uff08\u6216\u8005\u752820\u6beb\u5347\u9488\u7ba1\uff09\u62bd\u53d6\u751f\u7406\u76d0\u6c344\u6beb\u5347\uff0c\u6253\u5165200\u4e07\u5355\u4f4d\u7684\u9752\u9709\u7d20\u74f6\u4e2d\uff0c\u628a\u9752\u9709\u7d20\u878d\u5316\uff0c\u7136\u540e\u75281\u6beb\u5347\u76ae\u8bd5\u9488\u7ba1\u62bd\u53d60.1\u6beb\u5347\u9752\u9709\u7d20\uff0c\u8fd9\u6837\u5c31\u7b49\u4e8e\u4f60\u62bd\u53d6\u4e86200\u4e07\u5355\u4f4d\u76841\uff0f40.
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\u6211\u4eec\u518d\u628a\u8fd90.1\u6beb\u5347\u7528\u751f\u7406\u76d0\u6c34\u6269\u5bb9\u52301\u6beb\u5347,\u518d\u6253\u51fa0.9\u6beb\u5347,\u662f\u4e0d\u662f\u53c8\u53d8\u6210\u4e861\uff0f40\u76841\uff0f10\u76841\uff0f10\u4e86\uff08\u5c31\u662f\u7b49\u5f0f\u6700\u540e\u8fb9\u76841\uff0f40\u00d71\uff0f10\u00d71\uff0f10\uff09\u3002
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绛旓細0.5ml婧舵恫绋閲10鍊嶅姞4.5ml鐨勬按銆灏0.5ml鐨勬憾娑茬█閲10鍊嶏紝灏辨槸灏嗗師鏉ョ殑0.5ml婧舵恫涓9鍊嶇殑姘存贩鍚堬紝涓鍏辨垚涓10鍊嶇█閲婄殑婧舵恫銆傞偅涔堥渶瑕佸姞鍏ョ殑姘寸殑浣撶Н鍙互閫氳繃浠ヤ笅鍏紡璁$畻寰楀埌锛氬姞姘翠綋绉=绋閲婂悗鎬讳綋绉-鍒濆婧舵恫浣撶Н=锛0.5ml*10锛-0.5ml=4.5ml銆
绛旓細绋閲婁簡100鍊嶃鍋囪鍘熸祿搴︿负a锛岄偅涔堝彇鍑0.5姣崌锛屾憾璐ㄧ殑閲忓氨鏄0.5锛岀█閲婂畬鎴愬悗锛屾祿搴﹀氨鍙樻垚0.5a闄や互50锛屽氨鏄痑/100 鎵浠ュ垰濂藉氨鏄█閲婁簡100鍊嶃
绛旓細鍏朵腑绋閲婂墠婧舵恫娴撳害闄や互绋閲婂悗鐨勬憾娑叉祿搴︽墍寰楃殑鍟嗘寜涓瀹氱殑姣斾緥锛绋閲婂嶆暟=鍘熸恫娴撳害/锛堝師娑叉祿搴γ楃Щ鍙栦綋绉/瀹氬浣撶Н锛銆傜█閲婂垎鏋 渚嬪鏈変竴鐡100mg/L鐨勬憾娑诧紝瑕佺█閲3鍊嶃5鍊嶃10鍊嶃20鍊嶃傛湁300姣崌鐨勫閲忕摱鎴栧叾浠栧彲瀹氬鐨勫鍣紝绋閲3鍊嶏紝鍗崇Щ鍙100mg/L鐨勬憾娑100mL锛屽畾瀹硅嚦300mL銆傚鏋滈渶瑕佺█閲5鍊嶏紝...
绛旓細閫氬父鎵璇寸殑绋閲婂嶆暟鏄竴绉嶇畝鍖栫殑璇存硶锛屽畠骞朵笉鑰冭檻瀹氬鐨勪綋绉傚鏋滆杩涜绮剧‘璁$畻锛搴斾娇鐢ㄤ互涓嬪叕寮忥細绋閲婂嶆暟 = 鍘熸恫娴撳害 / (鍘熸恫娴撳害 脳 绉诲彇浣撶Н / 瀹氬浣撶Н)銆3. 瀹為檯鎿嶄綔涓句緥锛氬亣璁句綘鏈変竴鐡舵祿搴︿负100mg/L鐨勬憾娑诧紝瑕佽繘琛屼笉鍚屽嶆暟鐨勭█閲婏紝姣斿3鍊嶃5鍊嶃10鍊嶃20鍊嶏紝骞朵笖浣跨敤涓涓300姣崌鐨勫...
绛旓細涓鍏辩敤鍘熸牱0.5ml锛鍔犳按1.5ml锛岀█閲鍊嶆暟=0.5+1.5/0.5=4.
绛旓細绋閲婂嶆暟鍙互閫氳繃浠ヤ笅鍏紡鏉ヨ绠楋細D = V1 / V2 鍏朵腑锛欴 鏄█閲婂嶆暟銆俈1 鏄垵濮嬫祿搴﹁緝楂樼殑婧舵恫鐨勪綋绉俈2 鏄姞鍏ョ殑绾憾鍓傜殑浣撶Н銆備緥濡傦紝濡傛灉浣犳湁50姣崌鐨勬祿搴︿负1鎽╁皵/鍗囩殑婧舵恫锛岀劧鍚庡悜鍏朵腑娣诲姞50姣崌鐨勬按锛屼綘鍙互璁$畻绋閲鍊嶆暟濡備笅锛欴 = V1 / V2 D = 50 mL / 50 mL D = 1 杩欒〃绀轰綘灏...
绛旓細渚嬪鏈涓绋閲2鍊嶏紝鍘熸恫娴撳害涓1锛屽姞鍏1mL姘寸█閲婁箣鍚庢祿搴﹀彉涓0.5锛鎵浠ュ師娑叉祿搴/绋閲婂悗娴撳害=1/0.5=2锛屽嵆绋閲2鍊嶃備互涓婄畻娉曟槸涓嶄弗璋ㄧ殑绮楃暐绠楁硶锛屼弗璋ㄧ畻娉曠殑绋閲婂嶆暟搴旇鏄寜鐓у畾瀹瑰灏戞潵绠楃殑銆傜█閲婂嶆暟=鍘熸恫娴撳害/锛堝師娑叉祿搴γ楃Щ鍙栦綋绉/瀹氬浣撶Н锛変緥濡傦紝浣犳湁涓鐡100mg/L鐨勬憾娑诧紝瑕佺█閲3鍊嶃5鍊嶃...
绛旓細1. 绋閲鍊嶆暟鐨勮绠鏂规硶娑夊強鍒板皢楂樻祿搴︽憾娑查氳繃娣诲姞婧跺墏鑰岄檷浣庢祿搴︺傝繖涓繃绋嬮氬父绉颁负绋閲娿備緥濡傦紝瑕佸皢10 mL鐨1 mol/L HCl婧舵恫绋閲婅嚦0.5 mol/L锛屽彧闇鍔犲叆9 mL鐨勬按銆2. 绋閲婂嶆暟鐨勬暟瀛﹁〃杈惧紡涓猴細绋閲婂嶆暟 = 鍘熸恫娴撳害 / (鍘熸恫娴撳害 脳 绉诲彇浣撶Н / 瀹氬浣撶Н)銆傝繖涓鍏紡璇存槑浜嗙█閲婂墠鍚庢憾娑蹭腑婧惰川鐗╄川...
绛旓細鍦ㄨ繘琛岀簿纭璁$畻鏃讹紝浣跨敤鍏紡锛氱█閲婂嶆暟 = 鍘熸恫娴撳害 / (鍘熸恫娴撳害 脳 绉诲彇浣撶Н / 瀹氬浣撶Н)銆備緥濡傦紝濡傛灉浣犳湁涓涓祿搴︿负100mg/L鐨勬憾娑诧紝瑕佽繘琛3鍊嶃5鍊嶃10鍊嶃20鍊嶇殑绋閲婏紝鍏蜂綋鎿嶄綔濡備笅锛3鍊嶇█閲婏細鍙100mL鍘熸恫锛屽姞200mL姘达紝瀹氬鍒300mL銆 5鍊嶇█閲婏細鍙60mL鍘熸恫锛屽姞240mL姘达紝瀹氬鍒300mL銆
绛旓細灏嗚婧舵恫绋閲婂埌0.5ug/ml鐨勮绠鏂规硶濡備笅锛1銆侀鍏堬紝鍋囪闇瑕佸彇鍑篤1 mL鐨1ug/ml婧舵恫锛屽苟灏嗗叾鍔犲叆鍒癡2 mL鐨勭█閲婃恫涓紝浣垮緱鏈缁堟祿搴︿负0.5ug/ml銆2銆佹牴鎹憾娑茬殑绋閲婂叕寮锛欳1V1 =C2V2銆傚叾涓紝C1涓哄垵濮嬫憾娑茬殑娴撳害锛孷1涓哄彇鍑虹殑鍒濆婧舵恫鐨勪綋绉紝C2涓烘渶缁堟憾娑茬殑娴撳害锛孷2涓烘渶缁堟憾娑茬殑浣撶Н銆3銆佷唬鍏ユ暟鎹...