哪位高人帮忙翻译成英文 跪求!哪位高人能帮忙翻译成英文?在线等,谢谢!
\u54ea\u4f4d\u9ad8\u4eba\u5e2e\u5fd9\u7ffb\u8bd1\u6210\u82f1\u6587\uff0c\u6025\u7528\u6027\u8d3f\u8d42is now more concerned about the form of bribes, the social harm caused by the negative impact of society are increasing. In view of this, the question of whether to legislate\u6027\u8d3f\u8d42is always a matter of debate. In recent years, additional requirements in the criminal law "\u6027\u8d3f\u8d42crime" the voice of growing, both in theoretical circles or society, are of the view that legislation should be\u6027\u8d3f\u8d42has played strong, but a social behavior to rise to the height of the Legislative Council, the inevitable need to serious on the relevant legal and social reality to explore further. This article will support the views of the Legislative\u6027\u8d3f\u8d42reflection, discourse and argued for\u6027\u8d3f\u8d42not find a sufficient basis for legislation.
1 for each day one time limit. Tickets sold without replacement, vice roll to tear is invalid.
2 the old and young and must be accompanied by someone.
3 in the tour and watch visitors performance, please keep order, shall not vote, teasing and threaten animal violators accident responsibility.
4. Please use the library to feed in the specified area feed, Disable bring food or other sundry feed, lest and animal health.
5 please put garbage and waste bins, do not abandon. Everywhere
6 strictly carry inflammable, explosive, toxic substances and pet and other animals into the library.
7. Every child shall have one or more adults accompanied, Please take good care of your children, not in danger area, prohibit to play on the window glass.
8. Without permission, prohibited in the filming movies and advertising.
Expresses the system and the genetic transformation technology development along with the transgene, takes one bioreactor production for medicinal purposes protein using the transgenic plant to become in the plant genetic engineering new growing point [3,10]. present the extraneous source protein which expresses using the plant bioreactor mainly to have the transgenic plant vaccine, the transgenic plant immune body and the transgenic plant production protein class medicine. Has been able to succeed expresses has the hepatitis B virus surface antigen (HBSAg), the person hepatitis C virus antigen, secretion immunoglobulin A (SIgA), the interferon and the brain fei peptide and so on many kinds of protein [11,13]. The person leukemia inhibiting factor (LIF) at present only has the expression in the backwoods coli and the yeast, but expresses the system and the animal with the nucleus biology expresses the system to compare, the plant bioreactor is the most economical protein production system, can the large scale production protein, be advantageous stores, the transportation, moreover the plant has the complete eukaryotic cell to express the system, can carry on the accurate processing to the expression extraneous source protein, expresses the product nontoxicity and the side effect, safe reliable [14], but this gene's has not seen the report at present in plant expression research. Therefore this research constructed this gene plant the expression vector, changed over to the pattern plant tobacco, the screening masculine adult plant carried on Southern, the Northern hybrid and the protein activeness determination analysis, studied it in tobacco's expression situation, produced the LIF protein for this gene using the plant bioreactor to lay the foundation.
Leukemia inhibitory factor (LIF) is a kind of biological functions of a variety of cytokines, it originated in the research end of the 1970s [2]. LIF hematopoietic cells can promote the growth and proliferation, inhibited the growth of leukemia cells, and induction or synergistic induction of differentiation. years of research found that LIF and a number of other functions, and low concentrations of LIF myoblasts could stimulate cell growth, LIF at the same time can inhibit the activity of lipoprotein lipase to participate in fat metabolism and impede fat synthesis etc. As LIF a wide range of biological characteristics of the people in accordance with their characteristics corresponding to the names: D-factor, DIA, DIF, CNDF, HILDA, OAF, HSF-3, LLI, etc. [6,13]. originally only found leukemia inhibitory factor gene , but at that time experimental conditions and technical constraints has not been an in-depth study until 1988 Gongh such as from the gene library LIF isolated from the gene, intron fragment removed, in the 5 'and 3' respectively the introduction of restriction sites, building into yeast expression vector and its expression in the Gearing .1989 passing on the expression vector improvements, and finally in E. coli can be highly expressed in full biological activity of the LIF [9 ]. Chinese scientists study the expression of recombinant LIF abroad later than many, 1995 is the first successful cloning of Ching Ming Ding LIFcDNA, will be cloned to create expression vector pBV220 recombinant plasmid was transformed into Escherichia coli DH5 α, after induction in the LIF DH5 α expression in the capacity of 10% [1] .2001 Quanzhong, etc., will be 575-bp fragment of the gene in the human lif Pichia pastoris expression, and the natural product of the same size and LIF with biological activity, and total protein expression 32.8% [7].
With transgenic expression system and the development of genetic transformation technologies, the use of transgenic plants as a bioreactor production of medicinal plant genetic engineering has become a new growth point in [3,10]. Current bioreactor plants use the expression , a major source of protein transgenic plant vaccines, antibodies and transgenic plants transgenic plant production of protein drugs. has been able to have success in the hepatitis B virus surface antigen (HBSAg), human hepatitis C virus antigen, secretory immunoglobulin A (SIgA ), interferon and enkephalin, and other protein [11, 13]. human leukemia inhibitory factor (LIF) currently only in E. coli and yeast expression, and prokaryotic expression systems and expression system compared to animals, plants bioreactor is the most economical protein production system capable of large-scale production of proteins, to facilitate storage, transport, possess integrity of the plant and eukaryotic expression system capable of heterologous protein expression of the accurate processing, and the expression of non-toxic side-effects, safety and reliability [14], but the gene expression in plants is still not reported. Therefore, the study of the construction of the plant gene expression vector into the model plant tobacco, positive screening plants for Southern, Northern blot and the activity of protein analysis, and research in the expression of tobacco, the use of plant genetic bioreactor protein LIF lay the foundation for production.
太长了 而且有很多的专业名词
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