哪位高人帮忙翻译成英文 跪求!哪位高人能帮忙翻译成英文?在线等,谢谢!

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\u6027\u8d3f\u8d42is now more concerned about the form of bribes, the social harm caused by the negative impact of society are increasing. In view of this, the question of whether to legislate\u6027\u8d3f\u8d42is always a matter of debate. In recent years, additional requirements in the criminal law "\u6027\u8d3f\u8d42crime" the voice of growing, both in theoretical circles or society, are of the view that legislation should be\u6027\u8d3f\u8d42has played strong, but a social behavior to rise to the height of the Legislative Council, the inevitable need to serious on the relevant legal and social reality to explore further. This article will support the views of the Legislative\u6027\u8d3f\u8d42reflection, discourse and argued for\u6027\u8d3f\u8d42not find a sufficient basis for legislation.

1 for each day one time limit. Tickets sold without replacement, vice roll to tear is invalid.
2 the old and young and must be accompanied by someone.
3 in the tour and watch visitors performance, please keep order, shall not vote, teasing and threaten animal violators accident responsibility.
4. Please use the library to feed in the specified area feed, Disable bring food or other sundry feed, lest and animal health.
5 please put garbage and waste bins, do not abandon. Everywhere
6 strictly carry inflammable, explosive, toxic substances and pet and other animals into the library.
7. Every child shall have one or more adults accompanied, Please take good care of your children, not in danger area, prohibit to play on the window glass.
8. Without permission, prohibited in the filming movies and advertising.

白血病抑制因子(LIF)是一种具有多种生物学功能的细胞因子,对它的研究起源于70年代末期[2].LIF能够促进造血细胞的生长与增殖,使白血病细胞生长受到抑制,同时诱导或协同诱导其分化. 多年的研究发现LIF还有一些其他功能,低浓度的LIF能明显刺激成肌细胞生长,同时LIF还能抑制脂蛋白酶的活性,参与脂肪代谢,阻碍脂肪合成等.由于LIF广泛的生物学特性,人们根据其特点给予相应的命名:D-因子,DIA,DIF,CNDF,HILDA,OAF,HSF-3,LLI等[6,13]. 最初人们只是发现了白血病抑制因子基因,但由于当时实验条件和技术的限制一直没有进行深入的研究,直到1988年Gongh等从人的基因文库中分离出LIF的基因,除去内含子片段后,在5’端和3’端分别引入酶切位点,构建表达载体并导入酵母中使其表达.1989年Gearing等通过对上述表达载体的改进,终于在大肠杆菌中也可以高效表达具有完整生物学活性的人的LIF[9].我国科学家研究LIF基因重组表达情况较国外晚很多,最早是1995年丁清明等成功克隆LIFcDNA后,将其克隆至表达载体pBV220上形成重组质粒,并转化DH5α大肠杆菌,经过诱导LIF在DH5α中的表达量达10%[1].2001年王全忠等将575bp的人lif基因片段在巴斯德毕赤酵母中表达,产物与天然LIF大小相近且具有生物活性,表达量为总蛋白的32.8% [7].
Expresses the system and the genetic transformation technology development along with the transgene, takes one bioreactor production for medicinal purposes protein using the transgenic plant to become in the plant genetic engineering new growing point [3,10]. present the extraneous source protein which expresses using the plant bioreactor mainly to have the transgenic plant vaccine, the transgenic plant immune body and the transgenic plant production protein class medicine. Has been able to succeed expresses has the hepatitis B virus surface antigen (HBSAg), the person hepatitis C virus antigen, secretion immunoglobulin A (SIgA), the interferon and the brain fei peptide and so on many kinds of protein [11,13]. The person leukemia inhibiting factor (LIF) at present only has the expression in the backwoods coli and the yeast, but expresses the system and the animal with the nucleus biology expresses the system to compare, the plant bioreactor is the most economical protein production system, can the large scale production protein, be advantageous stores, the transportation, moreover the plant has the complete eukaryotic cell to express the system, can carry on the accurate processing to the expression extraneous source protein, expresses the product nontoxicity and the side effect, safe reliable [14], but this gene's has not seen the report at present in plant expression research. Therefore this research constructed this gene plant the expression vector, changed over to the pattern plant tobacco, the screening masculine adult plant carried on Southern, the Northern hybrid and the protein activeness determination analysis, studied it in tobacco's expression situation, produced the LIF protein for this gene using the plant bioreactor to lay the foundation.

Leukemia inhibitory factor (LIF) is a kind of biological functions of a variety of cytokines, it originated in the research end of the 1970s [2]. LIF hematopoietic cells can promote the growth and proliferation, inhibited the growth of leukemia cells, and induction or synergistic induction of differentiation. years of research found that LIF and a number of other functions, and low concentrations of LIF myoblasts could stimulate cell growth, LIF at the same time can inhibit the activity of lipoprotein lipase to participate in fat metabolism and impede fat synthesis etc. As LIF a wide range of biological characteristics of the people in accordance with their characteristics corresponding to the names: D-factor, DIA, DIF, CNDF, HILDA, OAF, HSF-3, LLI, etc. [6,13]. originally only found leukemia inhibitory factor gene , but at that time experimental conditions and technical constraints has not been an in-depth study until 1988 Gongh such as from the gene library LIF isolated from the gene, intron fragment removed, in the 5 'and 3' respectively the introduction of restriction sites, building into yeast expression vector and its expression in the Gearing .1989 passing on the expression vector improvements, and finally in E. coli can be highly expressed in full biological activity of the LIF [9 ]. Chinese scientists study the expression of recombinant LIF abroad later than many, 1995 is the first successful cloning of Ching Ming Ding LIFcDNA, will be cloned to create expression vector pBV220 recombinant plasmid was transformed into Escherichia coli DH5 α, after induction in the LIF DH5 α expression in the capacity of 10% [1] .2001 Quanzhong, etc., will be 575-bp fragment of the gene in the human lif Pichia pastoris expression, and the natural product of the same size and LIF with biological activity, and total protein expression 32.8% [7].
With transgenic expression system and the development of genetic transformation technologies, the use of transgenic plants as a bioreactor production of medicinal plant genetic engineering has become a new growth point in [3,10]. Current bioreactor plants use the expression , a major source of protein transgenic plant vaccines, antibodies and transgenic plants transgenic plant production of protein drugs. has been able to have success in the hepatitis B virus surface antigen (HBSAg), human hepatitis C virus antigen, secretory immunoglobulin A (SIgA ), interferon and enkephalin, and other protein [11, 13]. human leukemia inhibitory factor (LIF) currently only in E. coli and yeast expression, and prokaryotic expression systems and expression system compared to animals, plants bioreactor is the most economical protein production system capable of large-scale production of proteins, to facilitate storage, transport, possess integrity of the plant and eukaryotic expression system capable of heterologous protein expression of the accurate processing, and the expression of non-toxic side-effects, safety and reliability [14], but the gene expression in plants is still not reported. Therefore, the study of the construction of the plant gene expression vector into the model plant tobacco, positive screening plants for Southern, Northern blot and the activity of protein analysis, and research in the expression of tobacco, the use of plant genetic bioreactor protein LIF lay the foundation for production.

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