如何配置0.2mol/L的磷酸盐缓冲液（Ph=7.0） pH7.4的0.2 mol/L磷酸缓冲液怎么配制啊？ 谢谢喽
\u5982\u4f55\u7528KH2PO4\u548cK2HPO4\u914d\u5236pH=6.1\u7684\u78f7\u9178\u76d0\u7f13\u51b2\u6db21\u3001\u8fd0\u7528\u516c\u5f0f ph=pka-lg\uff08c\u9178\c\u78b1\uff09
2\u3001\u5728\u8fd9\u91cc,\u9178\u4e3aKH2PO4\u78b1\u4e3aK2HPO4pka\u4e3a\u78f7\u9178\u7684pka2ph=6.1\u4ee3\u5165\u5373\u53ef
3\u3001\u7f13\u51b2\u6db2\u6709\u6548\u7f13\u51b2\u8303\u56f4\u4e3aPH=PK\u00b11\u4e4b\u5185,\u5c31\u662f\u8bf4\u5171\u8f6d\u9178\u78b1\u7684\u6d53\u5ea6\u6bd4\u5e94\u8be5\u572810/1\u62161/10\u4e4b\u5185\uff0c\u9898\u76ee\u4e2d\u8ba1\u7b97\u9700\u8981\u7684PK2=7.2\u672c\u9898\u6eb6\u6db2\u7684PH=6.1\u4e0d\u5728\u8fd9\u4e2a\u8981\u6c42\u4e4b\u5185\u3002\u867d\u7136\u53ef\u4ee5\u914d\u5236\u8981\u6c42\u7684PH\u503c\uff0c\u4f46\u7f13\u51b2\u80fd\u529b\u6709\u9650\u3002\u6211\u4eec\u6765\u8ba1\u7b97\u4e00\u4e0b\u4e24\u8005\u7684\u6d53\u5ea6\u4e4b\u6bd4\u3002
4\u3001PH=PKa2-lg[KH2PO4]/[K2HPO4]5\u30011=7.2-lg[KH2PO4]/[K2HPO4]
6\u3001lg[KH2PO4]/[K2HPO4]=1.17\u3001[KH2PO4]/[K2HPO4]=12.6/18\u3001\u53ea\u8981KH2PO4\u4e0eK2HPO4\u7269\u8d28\u7684\u91cf\u6bd412.6:1\uff0c\u5c31\u53ef\u4ee5\u914d\u5236\u6210PH=6.1\u7684\u7f13\u51b2\u6eb6\u6db2\u3002
9\u3001\u4f46\u6211\u4eec\u770b\u5230lKH2PO4\u7684\u7269\u8d28\u7684\u91cf\u6d53\u5ea6\u662fK2HPO4\u768412.6\u500d\uff0c\u76f8\u5dee\u5de8\u5927\uff0c\u6240\u4ee5\u5e76\u975e\u7406\u60f3\u7684\u7f13\u51b2\u6eb6\u6db2\u3002
\u6269\u5c55\u8d44\u6599\uff1a
1\u3001\u78f7\u9178\u7f13\u51b2\u6db2\u7684\u7f13\u51b2pH\u503c\u8303\u56f4\u975e\u5e38\u5e7f\u6cdb\uff0c\u53ef\u914d\u7f6e\u5404\u79cdpH\u503c\u7684\u9178\u6027\u3001\u78b1\u6027\u548c\u4e2d\u6027\u7f13\u51b2\u6db2\uff0c\u914d\u5236\u9178\u6027\u7f13\u51b2\u6db2\u53ef\u76f4\u63a5\u7528NaH2PO4\u6216KH2PO4\uff0cpH\u8303\u56f4\u57281-5\u3002
2\u3001\u78b1\u6027\u7f13\u51b2\u6db2\u53ef\u76f4\u63a5\u7528Na2HPO4\u6216K2HPO4\uff0cpH\u8303\u56f4\u57289-12\uff1b\u4e2d\u6027\u7f13\u51b2\u6db2\u5219\u7528\u7b49\u6d53\u5ea6\u7684NaH2PO4\u548cNa2HPO4\u6216\u8005\u7b49\u6d53\u5ea6\u7684KH2PO4\u548cK2HPO4\u6eb6\u6db2\u6df7\u5408\uff0cpH\u57285.5-8.5\u3002
3\u3001\u6d53\u5ea6\u8ba1\u7b97\uff0c\u901a\u5e38\u6240\u8bf4\u7684\u78f7\u9178\u7f13\u51b2\u6db2\u7684\u6d53\u5ea6\u662f\u6307\u6eb6\u6db2\u4e2d\u542b\u6709\u7684\u6240\u6709\u7684\u78f7\u9178\u6839\u79bb\u5b50\u7684\u6d53\u5ea6\uff0c\u800c\u4e0d\u662f\u94a0\u79bb\u5b50\uff08Na+\uff09\u6216\u8005\u94be\u79bb\u5b50\uff08K+\uff09\u7684\u6d53\u5ea6\u3002
4\u3001\u5728\u914d\u5236\u4e2d\u6027\u7f13\u51b2\u6db2\u65f6\uff0c\u7528\u7b49\u6d53\u5ea6\u7684NaH2PO4\u548cNa2HPO4\u6216\u8005\u7b49\u6d53\u5ea6\u7684KH2PO4\u548cK2HPO4\u6eb6\u6db2\u6df7\u5408\uff0c\u5e76\u4e0d\u5f71\u54cd\u78f7\u9178\u6839\u79bb\u5b50\u7684\u6d53\u5ea6\uff0c\u6240\u5f97\u7684\u7f13\u51b2\u6db2\u6d53\u5ea6\u4ecd\u662f\u7b49\u6d53\u5ea6\u7684NaH2PO4\u548cNa2HPO4\u6216\u8005\u7b49\u6d53\u5ea6\u7684KH2PO4\u548cK2HPO4\u6eb6\u6db2\u539f\u6765\u7684\u6d53\u5ea6\uff0c\u4f46\u662f\u7f13\u51b2\u6db2\u4e2d\u7684\u94a0\u79bb\u5b50\uff08Na+\uff09\u6216\u8005\u94be\u79bb\u5b50\uff08K+\uff09\u7684\u6d53\u5ea6\u5df2\u7ecf\u53d1\u751f\u53d8\u5316\u3002
5\u3001\u4f18\u7f3a\u70b9\uff0c\u78f7\u9178\u7f13\u51b2\u6db2\u7684\u4f18\u70b9\uff1a\u6613\u914d\u5236\u5404\u79cd\u6d53\u5ea6\u7684\u7f13\u51b2\u6db2\uff1b\u9002\u7528\u7684pH\u503c\u8303\u56f4\u5bbd\uff0c\u53ef\u914d\u7f6e\u5404\u79cdpH\u503c\u7684\u7f13\u51b2\u6db2\uff1bpH\u53d7\u6e29\u5ea6\u5f71\u54cd\u8f83\u5c0f\uff1b\u7f13\u51b2\u80fd\u529b\u5f3a\uff0c\u7f13\u51b2\u6db2\u88ab\u7a00\u91ca\u540epH\u53d8\u5316\u5c0f\u3002
6\u3001\u78f7\u9178\u7f13\u51b2\u6db2\u7684\u7f3a\u70b9\uff1a\u6613\u4e0e\u5e38\u89c1\u7684\u9499\u79bb\u5b50Ca2+\u3001Mg2+\u53ca\u4e00\u4e9b\u91cd\u91d1\u5c5e\u79bb\u5b50\u7f14\u5408\u5f62\u6210\u6c89\u6dc0\uff1b\u4f1a\u6291\u5236\u67d0\u4e9b\u751f\u5316\u53cd\u5e94\u8fc7\u7a0b\uff0c\u5982\u6291\u5236\u67d0\u4e9b\u9176\u7684\u50ac\u5316\u8fc7\u7a0b\u7b49\u3002
\u5206\u522b\u914d\u52360.2mol/L\u7684Na2HPO4\u6eb6\u6db2\u548c0.2mol/L\u7684NaH2PO4\u6eb6\u6db2\uff08\u5177\u4f53\u6b65\u9aa4\uff1a\u79f0\u53d6Na2HPO4.12H2O \u6676\u4f537.16g\u6eb6\u89e3\uff0c\u7528100ml\u5bb9\u91cf\u74f6\u5b9a\u5bb9\uff0c\u5f970.2mol/L\u7684Na2HPO4\u6eb6\u6db2\uff0c\u79f0\u53d6NaH2PO4.2H2O\u6676\u4f533.12g\u6eb6\u89e3\uff0c\u7528100ml\u5bb9\u91cf\u74f6\u5b9a\u5bb9\uff0c\u5f970.2mol/L\u7684NaH2PO4\u6eb6\u6db2\uff09
\u53d60.2mol/L\u7684Na2HPO4\u6eb6\u6db281ml\uff0c\u518d\u53d60.2mol/L\u7684NaH2PO4\u6eb6\u6db219ml\u6df7\u5408\uff0c\u5373\u5f97pH7.4\u76840.2 mol/L\u78f7\u9178\u7f13\u51b2\u6db2
\u4e0d\u9700\u8981PH\u8ba1
取磷酸二氢钾0.68g，加0.1mol/L氢氧化钠溶液29.1mL，用水稀释到100mL，即得。
6.8g磷酸二氢钾，1.164g氢氧化钠，用水稀释至1000ml。
绛旓細0.2mol/l鑽夐吀婧舵恫閰嶇疆锛氬彇鑽夐吀12.8g鍔犳按閫傞噺浣挎憾瑙ｆ垚1000ml鍗冲彲銆傚噯纭噺鍙 35.00ml ~ 40.00ml 鑽夐吀婧舵恫浜 250ml 涓夎鐡朵腑锛屽姞 100ml H2SO4(8+92) 姘存憾娑层傜敤 c(1/5KMnO4) = 0.1 mol路L-1 楂橀敯閰搁捑鏍囧噯婧舵恫婊村畾杩戠粓鐐规椂锛屽姞鐑嚦 70鈩冿紝缁х画婊村畾鑷虫憾娑插憟绮夌孩鑹诧紝淇濇寔 30绉掞紝鍚屾椂鍋...
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绛旓細4锛岃繘琛屽疄闄呬笂鐨閰嶇疆锛岃繖闇瑕佸悇绉嶉噺鍏凤紝鍣ㄧ毧绛夛紝娉ㄦ剰閰嶅埗涓殑娉ㄦ剰浜嬮」锛屾敞鎰忓畨鍏ㄧ瓑銆傦紙鍥涳級婧舵恫闂存帴閰嶅埗鐨勬柟娉曚緥瑙 1锛岀█閲婃硶渚嬭В 渚嬶紙1锛夋祿搴︿负0.4mol/L鐨勭～閰搁摐婧舵恫100ml锛濡備綍绋閲婅嚦0.2mol/L锛熻В锛氬皢纭吀閾滄憾娑100ml杞叆200ml瀹归噺鐡朵腑锛屽啿娲楀師瀹瑰櫒锛屽皢鍐叉礂娑蹭竴骞惰浆鍏ワ紝鍔犳按鍒板埢搴︼紝鍧囧寑鍗虫垚...
绛旓細1銆mol/l鏄墿璐ㄧ殑閲忔祿搴︼紝鍥犳鐢ㄩ渶瑕閰嶇疆鐨勬祿搴︿箻浠ヨ鐗╄川鐨勬懇灏旇川閲忥紝鍦ㄥ姞鍏ュ搴旂殑姘存贩鍚堝潎鍖鍗冲彲銆2銆佽敆绯栫殑鍒嗗瓙閲忎负324.3锛岀敱浜庡彧閰嶇疆100ml钄楃硸0.9*324.3*0.1锛屽姞鍏100ml钂搁姘淬
绛旓細1銆佺洂閰告爣鍑嗘憾娑(0.2mol/L)鐨勯厤鍒 18.2ml 娴撶洂閰哥█閲婂畾瀹硅嚦1000ml,杞叆璇曞墏鐡,鎽囧寑,璐存爣绛.2銆佹爣瀹 锛1锛 绮惧瘑绉板彇鏃犳按 Na2CO3 涓変唤,姣忎唤 0.20~0.24g,鍒嗗埆缃簬涓変釜閿ュ舰鐡 涓.锛2锛 鍒嗗埆鍔犳按50ml ,浣挎憾瑙.锛3锛 鍔犵敳鍩烘鎸囩ず鍓 1 婊.锛4锛 鐢ㄧ洂閰告爣鍑嗘憾娑叉淮瀹氳嚦棰滆壊鐢遍粍鑹插彉涓烘鑹,...
绛旓細姝ラ:(1)璁＄畻:0.2mol/L*0.1L*58.5g/mol=1.2g(绮剧‘鍒板皬鏁扮偣鍚庝竴浣)(2)绉伴噺:鐢ㄦ墭鐩樺ぉ骞崇О姘寲閽1.2g(3)婧惰В:鍥轰綋鏀惧叆灏忕儳鏉,鍔犲皯閲忚捀棣忔按婧惰В,鐢ㄧ幓鐠冩鎼呮媽(4)鍐峰嵈:鑷冲娓(5)杞Щ:鐢ㄧ幓鐠冩寮曟祦杞叆100ML瀹归噺鐡(6)娲楁钉:鐢ㄨ捀棣忔按娲楁钉鐑ф澂,鐜荤拑妫2-3娆,娲楁钉娑蹭篃杞叆瀹归噺鐡(7)鎽囧姩:鎽囧姩...
绛旓細棣栧厛0.2M锛mol/L锛夋槸鎸 閱嬮吀閽犲姞涓婇唻閰哥殑mol/g涔嬪拰鎵浠ヨc锛圚Ac锛変负Xmol/L锛屽垯 c锛圢aAc锛変负锛0.2-X)mol/L.(娆查厤寰楁憾娑蹭腑)鐒跺悗锛岃繖涓や釜娴撳害涓嶈鍦ㄥ垵濮嬮厤鍒跺拰閰嶅緱婧舵恫涓』婊¤冻涓嬮潰鏂圭▼锛氫緥涓姹俻H=5.2锛孠a鏄疕AC涔欓吀鐨勮В绂诲父鏁帮紝鏌ヨ〃銆傛澶刾Ka=-logKa锛屽父鏁帮紝涓4.75鍒5.2=4.75+...
绛旓細3锛夎绠椼佺О閲忥紙鍩哄噯鐗╄川锛夐厤鍒0.2mol /L鐨勯偦鑻簩鐢查吀姘㈤捑l鏍囧噯婧舵恫锛4锛夌敤纰卞紡婊村畾绠℃淮瀹氶偦鑻簩鐢查吀姘㈤捑l鏍囧噯婧舵恫锛屽埌缁堢偣锛岃褰曟淮瀹氫綋绉紱5锛夎绠楅偦鑻簩鐢查吀姘㈤捑l鏍囧噯婧舵恫鐨勫噯纭祿搴︺傝嚦灏戝钩琛屾爣瀹4娆.锛6锛夎创濂芥爣鍑嗘憾娑叉爣绛俱傘2銆0.2mol /L鐨凥Cl鏍囧噯婧舵恫鐨閰嶇疆鏂规硶 1锛夊彲浠ュ厛璁＄畻閰嶅埗1000...
绛旓細楗卞拰鐨勪笁姘寲閾佹憾娑查ケ鍜屾祿搴︿负5.67mol/L 鍋囪瑕佸埗浣1000ml鐨0.2mol/l鐨勪笁姘寲閾 鍙寜涓嬪紡璁＄畻閲忓彇娴撶洂閰哥殑浣撶Н锛0.2*1000=5.67*V V=0.2*1000/5.67=35.3ml 閰嶇疆鏂规硶涓猴細鍙35.3ml楗卞拰涓夋隘鍖栭搧婧舵恫锛岀疆浜庡凡鐩涙湁閫傞噺钂搁姘寸殑1000姣崌鐨勫閲忕摱涓紝鐢ㄨ捀棣忔按绋閲婅嚦鍒诲害鎽囧寑銆傛隘鍖栭搧鏄竴绉嶅叡浠峰寲鍚堢墿...
绛旓細3锛夎绠椼佺О閲忥紙鍩哄噯鐗╄川锛夐厤鍒0.2mol /L鐨勯偦鑻簩鐢查吀姘㈤捑l鏍囧噯婧舵恫锛4锛夌敤纰卞紡婊村畾绠℃淮瀹氶偦鑻簩鐢查吀姘㈤捑l鏍囧噯婧舵恫,鍒扮粓鐐,璁板綍婊村畾浣撶Н锛5锛夎绠楅偦鑻簩鐢查吀姘㈤捑l鏍囧噯婧舵恫鐨勫噯纭祿搴.鑷冲皯骞宠鏍囧畾4娆.锛6锛夎创濂芥爣鍑嗘憾娑叉爣绛.銆2銆0.2mol /L鐨凥Cl鏍囧噯婧舵恫鐨閰嶇疆鏂规硶 1锛夊彲浠ュ厛璁＄畻閰嶅埗1000姣崌...
