微生物育种的自然选育 微生物育种技术有哪些
\u5de5\u4e1a\u5fae\u751f\u7269\u80b2\u79cd\u56fd\u81ea\u7136\u9009\u54ea\u4e2a\u5b66\u90e8\u5c31\u662f\u4e0d\u7ecf\u4eba\u5de5\u5904\u7406\uff0c\u5229\u7528\u5fae\u751f\u7269\u7684\u81ea\u7136\u7a81\u53d8\u8fdb\u884c\u83cc\u79cd\u9009\u80b2\u7684\u8fc7\u7a0b\u79f0\u4e3a\u81ea\u7136\u9009\u80b2\u3002\u8fd9\u7c7b\u7a81\u53d8\u6ca1\u6709\u4eba\u5de5\u53c2\u4e0e\u5e76\u975e\u662f\u6ca1\u6709\u539f\u56e0\u7684\uff0c\u4e00\u822c\u8ba4\u4e3a\u81ea\u7136\u7a81\u53d8\u6709\u4e24\u79cd\u539f\u56e0\u5f15\u8d77\uff0c\u5373\u591a\u56e0\u7d20\u4f4e\u5242\u91cf\u6548\u5e94\u548c\u4e92\u53d8\u5f02\u6784\u6548\u5e94\u3002\u6240\u8c13\u591a\u56e0\u7d20\u4f4e\u5242\u91cf\u6548\u5e94\uff0c\u662f\u6307\u5728\u81ea\u7136\u73af\u5883\u4e2d\u5b58\u5728\u7740\u4f4e\u5242\u91cf\u7684\u5b87\u5b99\u5c04\u7ebf\u3001\u5404\u79cd\u77ed\u6ce2\u8f90\u5c04\u3001\u4f4e\u5242\u91cf\u7684\u8bf1\u53d8\u7269\u8d28\u548c\u5fae\u751f\u7269\u81ea\u8eab\u4ee3\u8c22\u4ea7\u751f\u7684\u8bf1\u53d8\u7269\u8d28\u7b49\u4f5c\u7528\u5f15\u8d77\u7684\u7a81\u53d8\u3002\u4e92\u53d8\u5f02\u6784\u6548\u5e94\u662f\u6307\u56db\u79cd\u78b1\u57fa\u7b2c\u516d\u4f4d\u4e0a\u7684\u916e\u57fa\u6216\u6c28\u57fa\u7684\u77ac\u95f4\u53d8\u6784\uff0c\u4f1a\u5f15\u8d77\u78b1\u57fa\u7684\u9519\u914d[3]\u3002\u81ea\u7136\u7a81\u53d8\u53ef\u80fd\u4f1a\u4ea7\u751f\u4e24\u79cd\u622a\u7136\u4e0d\u540c\u7684\u7ed3\u679c\uff0c\u4e00\u79cd\u662f\u83cc\u79cd\u9000\u5316\u800c\u5bfc\u81f4\u76ee\u6807\u4ea7\u91cf\u6216\u8d28\u91cf\u4e0b\u964d\uff1b\u53e6\u4e00\u79cd\u662f\u5bf9\u751f\u4ea7\u6709\u76ca\u7684\u7a81\u53d8\u3002\u4e3a\u4e86\u4fdd\u8bc1\u751f\u4ea7\u6c34\u5e73\u7684\u7a33\u5b9a\u548c\u63d0\u9ad8\uff0c\u5e94\u7ecf\u5e38\u5730\u8fdb\u884c\u751f\u4ea7\u83cc\u79cd\u81ea\u7136\u9009\u80b2\uff0c\u4ee5\u6dd8\u6c70\u9000\u5316\u7684\uff0c\u9009\u51fa\u4f18\u826f\u7684\u83cc\u79cd\u3002
\u5728\u5de5\u4e1a\u751f\u4ea7\u4e0a\uff0c\u7531\u4e8e\u5404\u79cd\u6761\u4ef6\u56e0\u7d20\u7684\u5f71\u54cd\uff0c\u81ea\u7136\u7a81\u53d8\u662f\u7ecf\u5e38\u53d1\u751f\u7684\uff0c\u4e5f\u9020\u6210\u4e86\u751f\u4ea7\u6c34\u5e73\u7684\u6ce2\u52a8\uff0c\u6240\u4ee5\u6280\u672f\u4eba\u5458\u5f88\u6ce8\u610f\u4ece\u9ad8\u751f\u4ea7\u6c34\u5e73\u7684\u6279\u6b21\u4e2d\uff0c\u5206\u79bb\u9ad8\u751f\u4ea7\u80fd\u529b\u7684\u83cc\u79cd\u518d\u7528\u4e8e\u751f\u4ea7\u3002\u540c\u65f6\u4e5f\u53ef\u5229\u7528\u81ea\u53d1\u7a81\u53d8\u800c\u51fa\u73b0\u7684\u83cc\u79cd\u6027\u72b6\u7684\u53d8\u5316\uff0c\u53bb\u9009\u80b2\u4f18\u826f\u7684\u83cc\u682a\uff0c\u5982\u5728\u5473\u7cbe\u53d1\u9175\u88ab\u566c\u83cc\u4f53\u6c61\u67d3\u8fc7\u7a0b\u4e2d\uff0c\u6240\u9009\u51fa\u7684\u6297\u566c\u83cc\u4f53\u83cc\u682a\u3002\u81ea\u7136\u9009\u80b2\u662f\u4e00\u79cd\u7b80\u5355\u6613\u884c\u7684\u9009\u80b2\u65b9\u6cd5\uff0c\u53ef\u4ee5\u8fbe\u5230\u7eaf\u5316\u83cc\u79cd\uff0c\u9632\u6b62\u83cc\u79cd\u9000\u5316\uff0c\u7a33\u5b9a\u751f\u4ea7\uff0c\u63d0\u9ad8\u4ea7\u91cf\u7684\u76ee\u7684\u3002\u4f46\u662f\u81ea\u7136\u9009\u80b2\u7684\u6548\u7387\u4f4e\uff0c\u56e0\u6b64\u7ecf\u5e38\u8981\u4e0e\u8bf1\u53d8\u80b2\u79cd\u4ea4\u66ff\u4f7f\u7528\uff0c\u4ee5\u63d0\u9ad8\u80b2\u79cd\u6548\u7387\u3002
\u5176\u65b9\u6cd5\u901a\u5e38\u4e3a\u81ea\u7136\u9009\u80b2\u548c\u4eba\u5de5\u9009\u80b2\u4e24\u7c7b\uff0c\u53ef\u5355\u72ec\u4f7f\u7528\uff0c\u4e5f\u53ef\u4ea4\u53c9\u8fdb\u884c\u3002
DNA Shuffling\u6280\u672f
\u7f16\u8f91
\u968f\u7740PCR\u6280\u672f\u7684\u53d1\u5c55\u548c\u5e94\u7528\uff0c1994\u5e74\u7f8e\u56fd\u7684stemmer\u63d0\u51fa\u4e86\u4e00\u4e2a\u5168\u65b0\u7684\u4eba\u5de5\u5206\u5b50\u8fdb\u5316\u6280\u672f\u2014\u2014DNA Shuffling\uff08\u53c8\u79f0\u6d17\u724c\u6280\u672f\uff09\uff0c\u8be5\u6280\u672f\u80fd\u6a21\u62df\u751f\u7269\u5728\u6570\u767e\u5e74\u95f4\u53d1\u751f\u7684\u5206\u5b50\u8fdb\u5316\u8fc7\u7a0b\uff0c\u5e76\u53ef\u5728\u77ed\u7684\u5b9e\u9a8c\u5faa\u73af\u4e2d\u5b9a\u5411\u7b5b\u9009\u51fa\u7279\u5b9a\u57fa\u56e0\u7f16\u7801\u7684\u9176\u86cb\u767d\u6d3b\u6027\u63d0\u9ad8\u51e0\u767e\u500d\u751a\u81f3\u4e0a\u4e07\u500d\u7684\u529f\u80fd\u6027\u7a81\u53d8\u57fa\u56e0\u3002\u5176\u57fa\u672c\u539f\u7406\u662f\u5c06\u6765\u6e90\u4e0d\u540c\u4f46\u529f\u80fd\u76f8\u540c\u7684\u4e00\u7ec4\u540c\u6e90\u57fa\u56e0\uff0c\u7528DNA\u6838\u9178\u9176I\u8fdb\u884c\u6d88\u5316 \u4ea7\u751f\u968f\u673a\u5c0f\u7247\u6bb5\uff0c\u7531\u8fd9\u4e9b\u5c0f\u7247\u6bb5\u7ec4\u6210\u4e00\u4e2a\u6587\u5e93\uff0c\u4f7f\u4e4b\u4e92\u4e3a\u5f15\u7269\u548c\u6a21\u677f\uff0c\u8fdb\u884cPCR\u6269\u589e\uff0c\u5f53\u4e00\u4e2a\u57fa\u56e0\u62f7\u8d1d\u7247\u6bb5\u4f5c\u4e3a\u53e6\u4e00\u4e2a\u57fa\u56e0\u62f7\u8d1d\u7684\u5f15\u7269\u65f6\uff0c\u5f15\u8d77\u6a21\u677f\u8f6c\u6362\uff0c\u91cd\u7ec4\u56e0\u800c\u53d1\u751f\uff0c\u5bfc\u5165\u4f53\u5185\u540e\uff0c\u9009\u62e9\u6b63\u7a81\u53d8\u4f53\u4f5c\u65b0\u4e00\u8f6e\u7684\u4f53\u5916\u91cd\u7ec4\u3002\u4e00\u822c\u901a\u8fc72-3\u6b21\u5faa\u73af\uff0c\u8bfe\u83b7\u5f97\u4ea7\u7269\u5927\u5e45\u5ea6\u63d0\u9ad8\u7684\u91cd\u7ec4\u7a81\u53d8\u4f53\u3002
2\u81ea\u7136\u9009\u80b2
\u7f16\u8f91
\u5bf9\u81ea\u7136\u754c\u4e2d\u7684\u5fae\u751f\u7269\uff0c\u5728\u672a\u7ecf\u4eba\u5de5\u8bf1\u53d8\u6216\u6742\u4ea4\u5904\u7406\u7684\u60c5\u51b5\u4e0b\u8fdb\u884c\u5206\u79bb\u548c\u7eaf\u5316\uff08\u89c1\u5fae\u751f\u7269\u7684\u5206\u79bb\u548c\u7eaf\u5316\uff09\uff0c\u7136\u540e\u8fdb\u884c\u7eaf\u57f9\u517b\u548c\u6d4b\u5b9a\uff08\u89c1\u5fae\u751f\u7269\u6d4b\u5b9a\u6cd5\uff09\uff0c\u62e9\u4f18\u9009\u53d6\u5fae\u751f\u7269\u7684\u83cc\u79cd\u3002\u8fd9\u79cd\u65b9\u6cd5\u7b80\u5355\u6613\u884c\uff0c\u4f46\u83b7\u5f97\u4f18\u826f\u83cc\u79cd\u7684\u51e0\u7387\u5c0f\uff0c\u4e00\u822c\u96be\u4ee5\u6ee1\u8db3\u751f\u4ea7\u7684\u9700\u8981\u3002
3\u4eba\u5de5\u9009\u80b2
\u7f16\u8f91
\u5206\u8bf1\u53d8\u80b2\u79cd\u548c\u6742\u4ea4\u80b2\u79cd\u4e24\u79cd\u3002
\u8bf1\u53d8\u80b2\u79cd
\u4ee5\u8bf1\u53d1\u57fa\u56e0\u7a81\u53d8\u4e3a\u624b\u6bb5\u7684\u5fae\u751f\u7269\u80b2\u79cd\u6280\u672f\u30021927\u5e74\uff0cH.J. \u9a6c\u52d2\u53d1\u73b0X\u5c04\u7ebf\u6709\u589e\u52a0\u7a81\u53d8\u7387\u7684\u6548\u679c\uff1b1944\u5e74\uff0cC.\u5965\u5c14\u5df4\u514b\u9996\u6b21\u53d1\u73b0\u6c2e\u82a5\u5b50\u6c14\u7684\u8bf1\u53d8\u6548\u5e94\uff1b\u968f\u540e\uff0c\u4eba\u4eec\u9646\u7eed\u53d1\u73b0\u8bb8\u591a\u7269\u7406\u7684\uff08\u5982\u7d2b\u5916\u7ebf\u3001\u03b3\u5c04\u7ebf\u3001\u5feb\u4e2d\u5b50\u7b49\uff09\u548c\u5316\u5b66\u7684\u8bf1\u53d8\u56e0\u7d20\u3002\u5316\u5b66\u8bf1\u53d8\u56e0\u7d20\u5206\u4e3a3\u79cd\uff1a\u2460\u8bf1\u53d8\u5242\u4e0e\u4e00\u4e2a\u6216\u591a\u4e2a\u6838\u9178\u78b1\u57fa\u53d1\u751f\u5316\u5b66\u53d8\u5316\uff0c\u4f7fDNA\u590d\u5236\u65f6\u78b1\u57fa\u7f6e\u6362\u800c\u5f15\u8d77\u53d8\u5f02\uff0c\u5982\u7f9f\u80fa\u4e9a\u785d\u9178\u3001\u786b\u9178\u4e8c\u4e59\u916f\u3001\u7532\u57fa\u78fa\u9178\u4e59\u916f\u3001\u785d\u57fa\u80cd\u3001\u4e9a\u785d\u57fa\u7532\u57fa\u8132\u7b49\uff1b\u2461\u8bf1\u53d8\u5242\u662f\u5929\u7136\u78b1\u57fa\u7684\u7ed3\u6784\u7c7b\u4f3c\u7269\uff0c\u5728\u590d\u5236\u65f6\u53c2\u5165DNA\u5206\u5b50\u4e2d\u5f15\u8d77\u53d8\u5f02\uff0c\u59825-\u6eb4\u5c3f\u5627\u5576\u30015-\u6c28\u57fa\u5c3f\u5627\u5576\u30018-\u6c2e\u9e1f\u560c\u5464\u548c2-\u6c28\u57fa\u560c\u5464\u7b49\uff1b\u2462\u8bf1\u53d8\u5242\u5728DNA\u5206\u5b50\u4e0a\u51cf\u5c11\u6216\u589e\u52a01\uff5e2\u4e2a\u78b1\u57fa\uff0c\u4f7f\u78b1\u57fa\u7a81\u53d8\u70b9\u4ee5\u4e0b\u5168\u90e8\u9057\u4f20\u5bc6\u7801\u7684\u8f6c\u5f55\u548c\u7ffb\u8bd1\u53d1\u751f\u9519\u8bef\uff0c\u4ece\u800c\u5bfc\u81f4\u7801\u7ec4\u79fb\u52a8\u7a81\u53d8\u4f53\u7684\u51fa\u73b0\uff0c\u5982\u5416\u5576\u7c7b\u7269\u8d28\u548c\u4e00\u4e9b\u6c2e\u82a5\u884d\u751f\u7269\uff08ICR\uff09\u7b49\u3002\u8bf1\u53d8\u80b2\u79cd\u64cd\u4f5c\u7b80\u4fbf\uff0c\u7a81\u53d8\u7387\u9ad8\uff0c\u7a81\u53d8\u8c31\u5e7f\uff0c\u5b83\u4e0d\u4ec5\u80fd\u63d0\u9ad8\u4ea7\u91cf\uff0c\u6539\u8fdb\u8d28\u91cf\uff0c\u8fd8\u53ef\u6269\u5927\u4ea7\u54c1\u54c1\u79cd\u548c\u7b80\u5316\u5de5\u827a\u6761\u4ef6\u3002\u59821943\u5e74\u4ece\u81ea\u7136\u754c\u5206\u79bb\u5230\u7684\u9752\u9709\u7d20\u4ea7\u751f\u83cc\u7684\u6548\u4ef7\u53ea\u670920\u5355\u4f4d/\u6beb\u5347\uff0c\u7ecf\u8fc7\u4e00\u7cfb\u5217\u7684\u8bf1\u53d8\u80b2\u79cd\u540e\uff0c\u6548\u4ef7\u5df2\u8fbe40000\u5355\u4f4d/\u6beb\u5347\uff1b\u91d1\u9709\u7d20\u4ea7\u751f\u83cc\u7ecf\u8bf1\u53d8\u540e\uff0c\u53d1\u9175\u6db2\u4e2d\u53c8\u79ef\u7d2f\u4e86\u53bb\u7532\u57fa\u91d1\u9709\u7d20\uff1b\u8c37\u6c28\u9178\u68d2\u6746\u83cc1299\u7ecf\u7d2b\u5916\u7ebf\u8bf1\u53d8\u540e\uff0c\u6709\u7684\u80fd\u4ea7\u8d56\u6c28\u9178\uff0c\u6709\u7684\u80fd\u4ea7\u7f2c\u6c28\u9178\uff0c\u589e\u52a0\u4e86\u4ea7\u54c1\u7684\u79cd\u7c7b\uff1b\u571f\u9709\u7d20\u4ea7\u751f\u83cc\u7ecf\u8bf1\u53d8\u540e\uff0c\u9009\u5230\u4e86\u80fd\u51cf\u5c11\u6ce1\u6cab\u7684\u7a81\u53d8\u83cc\u682a\uff0c\u4ece\u800c\u63d0\u9ad8\u4e86\u53d1\u9175\u7f50\u7684\u5229\u7528\u7387\u3002\u8bf1\u53d8\u80b2\u79cd\u7684\u4e0d\u8db3\u662f\u7f3a\u4e4f\u5b9a\u5411\u6027\u3002
\u6742\u4ea4\u80b2\u79cd
\u4e0d\u540c\u57fa\u56e0\u578b\u7684\u54c1\u7cfb\u6216\u79cd\u5c5e\u95f4\uff0c\u901a\u8fc7\u4ea4\u914d\u6216\u4f53\u7ec6\u80de\u878d\u5408\u7b49\u624b\u6bb5\u5f62\u6210\u6742\u79cd\uff0c\u6216\u8005\u662f\u901a\u8fc7\u8f6c\u5316\u548c\u8f6c\u5bfc\u5f62\u6210\u91cd\u7ec4\u4f53\uff0c\u518d\u4ece\u8fd9\u4e9b\u6742\u79cd\u6216\u91cd\u7ec4\u4f53\u6216\u662f\u5b83\u4eec\u7684\u540e\u4ee3\u4e2d\u7b5b\u9009\u4f18\u826f\u83cc\u79cd\u3002\u901a\u8fc7\u8fd9\u79cd\u65b9\u6cd5\u53ef\u4ee5\u5206\u79bb\u5230\u5177\u6709\u65b0\u7684\u57fa\u56e0\u7ec4\u5408\u7684\u91cd\u7ec4\u4f53\uff0c\u4e5f\u53ef\u4ee5\u9009\u51fa\u7531\u4e8e\u5177\u6709\u6742\u79cd\u4f18\u52bf\u800c\u751f\u957f\u65fa\u76db\u3001\u751f\u7269\u91cf\u591a\u3001\u9002\u5e94\u6027\u5f3a\u4ee5\u53ca\u67d0\u4e9b\u9176\u6d3b\u6027\u63d0\u9ad8\u7684\u65b0\u54c1\u7cfb\u3002\u6742\u4ea4\u80b2\u79cd\u7684\u65b9\u5f0f\u56e0\u5b9e\u9a8c\u83cc\u682a\u7684\u751f\u6b96\u65b9\u5f0f\u4e0d\u540c\u800c\u5f02\uff0c\u5982\u6709\u6027\u6742\u4ea4\u3001\u51c6\u6027\u91cd\u7ec4\u3001\u539f\u751f\u8d28\u4f53\u878d\u5408\u3001\u8f6c\u5316\u3001\u8f6c\u5bfc\u3001\u6742\u79cd\u8d28\u7c92\u7684\u8f6c\u5316\u7b49\uff1b\u4f46\u662f\uff0c\u9009\u62e9\u4eb2\u682a\u3001\u5206\u79bb\u7fa4\u4f53\u540e\u4ee3\u7684\u57f9\u517b\u3001\u62e9\u4f18\u53bb\u52a3\u548c\u6742\u79cd\u9057\u4f20\u5206\u6790\u7684\u8fc7\u7a0b\u57fa\u672c\u662f\u76f8\u540c\u7684\u3002\u6742\u4ea4\u6cd5\u4e00\u822c\u6307\u6709\u4ea4\u914d\u53cd\u5e94\u7684\u83cc\u682a\u8fdb\u884c\u4ea4\u914d\u6216\u63a5\u5408\u800c\u5f62\u6210\u6742\u79cd\u3002\u8fd9\u79cd\u65b9\u6cd5\u9002\u7528\u8303\u56f4\u5f88\u5e7f\uff0c\u5728\u9152\u7c7b\u3001\u9762\u5305\u3001\u836f\u7528\u548c\u9972\u6599\u9175\u6bcd\u7684\u80b2\u79cd\uff0c\u94fe\u9709\u83cc\u548c\u9752\u9709\u83cc\u6297\u751f\u7d20\u4ea7\u91cf\u7684\u63d0\u9ad8\uff0c\u66f2\u9709\u7684\u9176\u6d3b\u6027\u589e\u5f3a\u7b49\u65b9\u9762\u5747\u5df2\u83b7\u5f97\u6210\u529f\u3002
\u4f53\u7ec6\u80de\u878d\u5408\u662f\u5728\u4e0d\u5177\u6027\u53cd\u5e94\u7684\u54c1\u7cfb\u6216\u79cd\u5c5e\u95f4\u7ec6\u80de\u878d\u5408\u548c\u67d3\u8272\u4f53\u91cd\u7ec4\uff0c\u5148\u7528\u9176\u6eb6\u89e3\u7ec6\u80de\u58c1\uff0c\u518d\u7528\u6c2f\u5316\u9499-\u805a\u4e59\u4e8c\u9187\u5904\u7406\u539f\u751f\u8d28\u4f53\uff0c\u4fc3\u4f7f\u878d\u5408\uff0c\u83b7\u5f97\u6742\u79cd\u3002\u6b64\u6cd5\u5728\u5de5\u4e1a\u5fae\u751f\u7269\u7684\u83cc\u79cd\u6539\u826f\u4e2d\u6709\u79ef\u6781\u4f5c\u7528\u3002
\u8f6c\u5316\u548c\u8f6c\u5bfc\u9996\u5148\u5e94\u7528\u4e8e\u7ec6\u83cc\uff0c\u73b0\u5df2\u5e7f\u6cdb\u7528\u4e8e\u94fe\u9709\u83cc\u548c\u9175\u6bcd\u83cc\u7b49\u3002\u968f\u7740\u91cd\u7ec4DNA\u6280\u672f\u7684\u53d1\u5c55\uff0c\u91cd\u7ec4\u8d28\u7c92\u7684\u6784\u5efa\u548c\u8f6c\u5316\u7cfb\u7edf\u7684\u786e\u7acb\uff0c\u5df2\u53ef\u5c06\u76ee\u7684\u57fa\u56e0\u8f6c\u79fb\u5230\u53d7\u4f53\u7ec6\u80de\u5185\uff0c\u5f97\u5230\u80fd\u4ea7\u751f\u5177\u6709\u91cd\u8981\u7ecf\u6d4e\u4ef7\u503c\u7684\u751f\u7269\u6d3b\u6027\u7269\u8d28\uff08\u5982\u75ab\u82d7\u3001\u9176\u7b49\uff09\u7684\u682a\u7cfb\u3002
\u5fae\u751f\u7269\u4e0e\u917f\u9020\u5de5\u4e1a\u3001\u98df\u54c1\u5de5\u4e1a\u3001\u751f\u7269\u5236\u54c1\u5de5\u4e1a\u7b49\u7684\u5173\u7cfb\u975e\u5e38\u5bc6\u5207\uff0c\u5176\u83cc\u682a\u7684\u4f18\u826f\u4e0e\u5426\u76f4\u63a5\u5173\u7cfb\u5230\u591a\u79cd\u5de5\u4e1a\u4ea7\u54c1\u7684\u597d\u574f\uff0c\u751a\u81f3\u5f71\u54cd\u4eba\u4eec\u7684\u65e5\u5e38\u751f\u6d3b\u8d28\u91cf\uff0c\u6240\u4ee5\u57f9\u80b2\u4f18\u8d28\u3001\u9ad8\u4ea7\u7684\u5fae\u751f\u7269\u83cc\u682a\u5341\u5206\u5fc5\u8981\u3002\u5fae\u751f\u7269\u80b2\u79cd\u7684\u76ee\u7684\u5c31\u662f\u8981\u628a\u751f\u7269\u5408\u6210\u7684\u4ee3\u8c22\u9014\u5f84\u671d\u4eba\u4eec\u6240\u5e0c\u671b\u7684\u65b9\u5411\u52a0\u4ee5\u5f15\u5bfc\uff0c\u6216\u8005\u4fc3\u4f7f\u7ec6\u80de\u5185\u53d1\u751f\u57fa\u56e0\u7684\u91cd\u65b0\u7ec4\u5408\u4f18\u5316\u9057\u4f20\u6027\u72b6\uff0c\u4eba\u4e3a\u5730\u4f7f\u67d0\u4e9b\u4ee3\u8c22\u4ea7\u7269\u8fc7\u91cf\u79ef\u7d2f\uff0c\u83b7\u5f97\u6240\u9700\u8981\u7684\u9ad8\u4ea7\u3001\u4f18\u8d28\u548c\u4f4e\u8017\u7684\u83cc\u79cd\u3002\u4f5c\u4e3a\u9014\u5f84\u4e4b\u4e00\u7684\u8bf1\u53d8\u80b2\u79cd\u4e00\u76f4\u88ab\u5e7f\u6cdb\u5e94\u7528\u3002\u76ee\u524d\uff0c\u56fd\u5185\u5fae\u751f\u7269\u80b2\u79cd\u754c\u4e3b\u8981\u91c7\u7528\u7684\u4ecd\u662f\u5e38\u89c4\u7684\u7269\u7406\u53ca\u5316\u5b66\u56e0\u5b50\u7b49\u8bf1\u53d8\u65b9\u6cd5\u3002\u6b64\u5916\uff0c\u539f\u751f\u8d28\u4f53\u8bf1\u53d8\u6280\u672f\u5df2\u5e7f\u6cdb\u5730\u5e94\u7528\u4e8e\u9176\u5236\u5242\u3001\u6297\u751f\u7d20\u3001\u6c28\u57fa\u9178\u3001\u7ef4\u751f\u7d20\u7b49\u7684\u83cc\u79cd\u9009\u80b2\u4e2d\uff0c\u5e76\u4e14\u53d6\u5f97\u4e86\u8bb8\u591a\u6709\u91cd\u5927\u5e94\u7528\u610f\u4e49\u7684\u6210\u679c\u3002
4\u8bf1\u53d8\u80b2\u79cd
\u7f16\u8f91
1.1\u7269\u7406\u8bf1\u53d8
1.1.1\u7d2b\u5916\u7167\u5c04
\u7d2b\u5916\u7ebf\u7167\u5c04\u662f\u5e38\u7528\u7684\u7269\u7406\u8bf1\u53d8\u65b9\u6cd5\u4e4b\u4e00\uff0c\u662f\u8bf1\u53d1\u5fae\u751f\u7269\u7a81\u53d8\u7684\u4e00\u79cd\u975e\u5e38\u6709\u7528\u7684\u5de5\u5177\u3002DNA \u548cRNA \u7684\u560c\u5464\u548c\u5627\u5576\u6700\u5927\u7684\u5438\u6536\u5cf0\u5728260nm\uff0c\u56e0\u6b64\u5728260nm \u7684\u7d2b\u5916\u8f90\u5c04\u662f\u6700\u6709\u6548\u7684\u81f4\u6b7b\u5242\u3002\u7d2b\u5916\u8f90\u5c04\u7684\u4f5c\u7528\u5df2\u6709\u591a\u79cd\u89e3\u91ca\uff0c\u4f46\u6bd4\u8f83\u786e\u5b9a\u7684\u4f5c\u7528\u662f\u4f7fDNA \u5206\u5b50\u5f62\u6210\u5627\u5576\u4e8c\u805a\u4f53[1]\u3002\u4e8c\u805a\u4f53\u7684\u5f62\u6210\u4f1a\u963b\u788d\u78b1\u57fa\u95f4\u6b63\u5e38\u914d\u5bf9\uff0c\u6240\u4ee5\u53ef\u80fd\u5bfc\u81f4\u7a81\u53d8\u751a\u81f3\u6b7b\u4ea1[2]\u3002
\u7d2b\u5916\u7167\u5c04\u8bf1\u53d8\u64cd\u4f5c\u7b80\u5355\uff0c\u7ecf\u6d4e\u5b9e\u60e0\uff0c\u4e00\u822c\u5b9e\u9a8c\u5ba4\u6761\u4ef6\u90fd\u53ef\u4ee5\u8fbe\u5230\uff0c\u4e14\u51fa\u73b0\u6b63\u7a81\u53d8\u7684\u51e0\u7387\u8f83\u9ad8\uff0c\u9175\u6bcd\u83cc\u682a\u7684\u8bf1\u53d8\u5927\u591a\u91c7\u7528\u8fd9\u79cd\u65b9\u6cd5\u3002
1.1.2\u7535\u79bb\u8f90\u5c04
\u03b3- \u5c04\u7ebf\u662f\u7535\u79bb\u751f\u7269\u5b66\u4e0a\u5e94\u7528\u6700\u5e7f\u6cdb\u7684\u7535\u79bb\u5c04\u7ebf\u4e4b\u4e00\uff0c\u5177\u6709\u5f88\u9ad8\u7684\u80fd\u91cf\uff0c\u80fd\u4ea7\u751f\u7535\u79bb\u4f5c\u7528,\u53ef\u76f4\u63a5\u6216\u95f4\u63a5\u5730\u6539\u53d8DNA \u7ed3\u6784\u3002\u5176\u76f4\u63a5\u6548\u5e94\u662f\u53ef\u4ee5\u6c27\u5316\u8131\u6c27\u6838\u7cd6\u7684\u78b1\u57fa\uff0c\u6216\u8005\u8131\u6c27\u6838\u7cd6\u7684\u5316\u5b66\u952e\u548c\u7cd6- \u78f7\u9178\u76f8\u8fde\u63a5\u7684\u5316\u5b66\u952e\u3002\u5176\u95f4\u63a5\u6548\u5e94\u662f\u80fd\u4f7f\u6c34\u6216\u6709\u673a\u5206\u5b50\u4ea7\u751f\u81ea\u7531\u57fa\uff0c\u8fd9\u4e9b\u81ea\u7531\u57fa\u53ef\u4ee5\u4e0e\u7ec6\u80de\u4e2d\u7684\u6eb6\u8d28\u5206\u5b50\u53d1\u751f\u5316\u5b66\u53d8\u5316\uff0c\u5bfc\u81f4DNA \u5206\u7f3a\u5931\u548c\u635f\u4f24[2]\u3002
\u9664\u03b3- \u5c04\u7ebf\u5916\u7684\u7535\u79bb\u8f90\u5c04\u8fd8\u6709X- \u5c04\u7ebf\u3001\u03b2- \u5c04\u7ebf\u548c\u5feb\u4e2d\u5b50\u7b49\u3002\u7535\u79bb\u8f90\u5c04\u6709\u4e00\u5b9a\u7684\u5c40\u9650\u6027\uff0c\u64cd\u4f5c\u8981\u6c42\u8f83\u9ad8\uff0c\u4e14\u6709\u4e00\u5b9a\u7684\u5371\u9669\u6027\uff0c\u901a\u5e38\u7528\u4e8e\u4e0d\u80fd\u4f7f\u7528\u5176\u4ed6\u8bf1\u53d8\u5242\u7684\u8bf1\u53d8\u80b2\u79cd\u8fc7\u7a0b\u3002
1.1.3\u79bb\u5b50\u6ce8\u5165
\u79bb\u5b50\u6ce8\u5165\u662f20 \u4e16\u7eaa80 \u5e74\u4ee3\u521d\u5174\u8d77\u7684\u4e00\u9879\u9ad8\u65b0\u6280\u672f\uff0c\u4e3b\u8981\u7528\u4e8e\u91d1\u5c5e\u6750\u6599\u8868\u9762\u7684\u6539\u6027\u30021986 \u5e74\u4ee5\u6765\u9010\u6e10\u7528\u4e8e\u519c\u4f5c\u7269\u80b2\u79cd\uff0c\u8fd1\u5e74\u6765\u5728\u5fae\u751f\u7269\u80b2\u79cd\u4e2d\u9010\u6e10\u5f15\u5165\u8be5\u6280\u672f[3]\u3002
\u79bb\u5b50\u6ce8\u5165\u65f6\uff0c\u751f\u7269\u5206\u5b50\u5438\u6536\u80fd\u91cf\uff0c\u5e76\u4e14\u5f15\u8d77\u590d\u6742\u7684\u7269\u7406\u548c\u5316\u5b66\u4e0a\u7684\u53d8\u5316\uff0c\u8fd9\u4e9b\u53d8\u5316\u7684\u4e2d\u95f4\u4f53\u662f\u5404\u7c7b\u6d3b\u6027\u81ea\u7531\u57fa\u3002\u8fd9\u4e9b\u81ea\u7531\u57fa\uff0c\u53ef\u4ee5\u5f15\u8d77\u5176\u5b83\u6b63\u5e38\u751f\u7269\u5206\u5b50\u7684\u635f\u4f24\uff0c\u53ef\u4f7f\u7ec6\u80de\u4e2d\u7684\u67d3\u8272\u4f53\u7a81\u53d8\uff0cDNA \u94fe\u65ad\u88c2\uff0c\u4e5f\u53ef\u4f7f\u8d28\u7c92DNA \u9020\u6210\u65ad\u88c2\u3002\u7531\u4e8e\u79bb\u5b50\u6ce8\u5165\u5c04\u7a0b\u5177\u6709\u53ef\u63a7\u6027\uff0c\u968f\u7740\u5fae\u675f\u6280\u672f\u548c\u7cbe\u786e\u5b9a\u4f4d\u6280\u672f\u7684\u53d1\u5c55\uff0c\u5b9a\u4f4d\u8bf1\u53d8\u5c06\u6210\u4e3a\u53ef\u80fd[4]\u3002
\u79bb\u5b50\u6ce8\u5165\u6cd5\u8fdb\u884c\u5fae\u751f\u7269\u8bf1\u53d8\u80b2\u79cd\uff0c\u4e00\u822c\u5b9e\u9a8c\u5ba4\u6761\u4ef6\u96be\u4ee5\u8fbe\u5230\uff0c\u76ee\u524d\u5e94\u7528\u76f8\u5bf9\u8f83\u5c11\u3002
1.1.4 \u6fc0\u5149
\u6fc0\u5149\u662f\u4e00\u79cd\u5149\u91cf\u5b50\u6d41\uff0c\u53c8\u79f0\u5149\u5fae\u7c92\u3002\u6fc0\u5149\u8f90\u5c04\u53ef\u4ee5\u901a\u8fc7\u4ea7\u751f\u5149\u3001\u70ed\u3001\u538b\u529b\u548c\u7535\u78c1\u573a\u6548\u5e94\u7684\u7efc\u5408\u5e94\u7528\uff0c\u76f4\u63a5\u6216\u95f4\u63a5\u5730\u5f71\u54cd\u6709\u673a\u4f53\uff0c\u5f15\u8d77\u7ec6\u80de\u67d3\u8272\u4f53\u7578\u53d8\u6548\u5e94\u3001\u9176\u7684\u6fc0\u6d3b\u6216\u949d\u5316\uff0c\u4ee5\u53ca\u7ec6\u80de\u5206\u88c2\u548c\u7ec6\u80de\u4ee3\u8c22\u6d3b\u52a8\u7684\u6539\u53d8\u3002\u5149\u91cf\u5b50\u5bf9\u7ec6\u80de\u5185\u542b\u7269\u4e2d\u7684\u4efb\u4f55\u7269\u8d28\u4e00\u65e6\u53d1\u751f\u4f5c\u7528\uff0c\u90fd\u53ef\u80fd\u5bfc\u81f4\u751f\u7269\u6709\u673a\u4f53\u5728\u7ec6\u80de\u5b66\u548c\u9057\u4f20\u5b66\u7279\u6027\u4e0a\u53d1\u751f\u53d8\u5f02\u3002\u4e0d\u540c\u79cd\u7c7b\u7684\u6fc0\u5149\u8f90\u5c04\u751f\u7269\u6709\u673a\u4f53\uff0c\u6240\u8868\u73b0\u51fa\u7684\u7ec6\u80de\u5b66\u548c\u9057\u4f20\u5b66\u53d8\u5316\u4e5f\u4e0d\u540c[5]\u3002
\u6fc0\u5149\u4f5c\u4e3a\u4e00\u79cd\u80b2\u79cd\u65b9\u6cd5\uff0c\u5177\u6709\u64cd\u4f5c\u7b80\u5355\u3001\u4f7f\u7528\u5b89\u5168\u7b49\u4f18\u70b9\uff0c\u8fd1\u5e74\u6765\u5e94\u7528\u4e8e\u5fae\u751f\u7269\u80b2\u79cd\u4e2d\u53d6\u5f97\u4e0d\u5c11\u8fdb\u5c55\u3002
1.1.5 \u5fae\u6ce2
\u5fae\u6ce2\u8f90\u5c04\u5c5e\u4e8e\u4e00\u79cd\u4f4e\u80fd\u7535\u78c1\u8f90\u5c04\uff0c\u5177\u6709\u8f83\u5f3a\u751f\u7269\u6548\u5e94\u7684\u9891\u7387\u8303\u56f4\u5728300MHz~300GHz\uff0c\u5bf9\u751f\u7269\u4f53\u5177\u6709\u70ed\u6548\u5e94\u548c\u975e\u70ed\u6548\u5e94\u3002\u5176\u70ed\u6548\u5e94\u662f\u6307\u5b83\u80fd\u5f15\u8d77\u751f\u7269\u4f53\u5c40\u90e8\u6e29\u5ea6\u4e0a\u5347\u3002\u4ece\u800c\u5f15\u8d77\u751f\u7406\u751f\u5316\u53cd\u5e94\uff1b\u975e\u70ed\u6548\u5e94\u6307\u5728\u5fae\u6ce2\u4f5c\u7528\u4e0b\uff0c\u751f\u7269\u4f53\u4f1a\u4ea7\u751f\u975e\u6e29\u5ea6\u5173\u8054\u7684\u5404\u79cd\u751f\u7406\u751f\u5316\u53cd\u5e94\u3002\u5728\u8fd9\u4e24\u79cd\u6548\u5e94\u7684\u7efc\u5408\u4f5c\u7528\u4e0b\uff0c\u751f\u7269\u4f53\u4f1a\u4ea7\u751f\u4e00\u7cfb\u5217\u7a81\u53d8\u6548\u5e94[6]\u3002
\u56e0\u800c,\u5fae\u6ce2\u4e5f\u88ab\u7528\u4e8e\u591a\u4e2a\u9886\u57df\u7684\u8bf1\u53d8\u80b2\u79cd\uff0c\u5982\u519c\u4f5c\u7269\u80b2\u79cd\u3001\u79bd\u517d\u80b2\u79cd\u548c\u5de5\u4e1a\u5fae\u751f\u7269\u80b2\u79cd\uff0c\u5e76\u53d6\u5f97\u4e86\u4e00\u5b9a\u6210\u679c\u3002
1.1.6 \u822a\u5929\u80b2\u79cd
\u822a\u5929\u80b2\u79cd\uff0c\u4e5f\u79f0\u7a7a\u95f4\u8bf1\u53d8\u80b2\u79cd\uff0c\u662f\u5229\u7528\u9ad8\u7a7a\u6c14\u7403\u3001\u8fd4\u56de\u5f0f\u536b\u661f\u3001\u98de\u8239\u7b49\u822a\u5929\u5668\u5c06\u4f5c\u7269\u79cd\u5b50\u3001\u7ec4\u7ec7\u3001\u5668\u5b98\u6216\u751f\u547d\u4e2a\u4f53\u642d\u8f7d\u5230\u5b87\u5b99\u7a7a\u95f4\uff0c\u5229\u7528\u5b87\u5b99\u7a7a\u95f4\u7279\u6b8a\u7684\u73af\u5883\u4f7f\u751f\u7269\u57fa\u56e0\u4ea7\u751f\u53d8\u5f02\uff0c\u518d\u8fd4\u56de\u5730\u9762\u8fdb\u884c\u9009\u80b2\uff0c\u57f9\u80b2\u65b0\u54c1\u79cd\u3001\u65b0\u6750\u6599\u7684\u4f5c\u7269\u80b2\u79cd\u65b0\u6280\u672f\u3002\u7a7a\u95f4\u73af\u5883\u56e0\u7d20\u4e3b\u8981\u6709\u5fae\u91cd\u529b\uff0c\u7a7a\u95f4\u8f90\u5c04\uff0c\u4ee5\u53ca\u5176\u5b83\u8bf1\u53d8\u56e0\u7d20\u5982\u4ea4\u53d8\u78c1\u573a\uff0c\u8d85\u771f\u7a7a\u73af\u5883\u7b49\uff0c\u8fd9\u4e9b\u56e0\u7d20\u4ea4\u4e92\u4f5c\u7528\u5bfc\u81f4\u751f\u7269\u7cfb\u7edf\u9057\u4f20\u7269\u7684\u635f\u4f24\uff0c\u4f7f\u751f\u7269\u53d1\u751f\u8bf8\u5982\u7a81\u53d8\u3001\u67d3\u8272\u4f53\u7578\u53d8\u3001\u7ec6\u80de\u5931\u6d3b\u3001\u53d1\u80b2\u5f02\u5e38\u7b49\u3002
\u822a\u5929\u80b2\u79cd\u8f83\u5176\u5b83\u80b2\u79cd\u65b9\u6cd5\u7279\u6b8a\uff0c\u662f\u822a\u5929\u6280\u672f\u4e0e\u5fae\u751f\u7269\u80b2\u79cd\u6280\u672f\u7684\u6709\u673a\u7ed3\u5408\uff0c\u6280\u672f\u542b\u91cf\u9ad8\uff0c\u6210\u672c\u9ad8\uff0c\u4e2a\u4f53\u7814\u7a76\u8005\u6216\u4e00\u822c\u7814\u7a76\u5355\u4f4d\u90fd\u96be\u4ee5\u5b9e\u73b0\uff0c\u53ea\u80fd\u4e0e\u822a\u5929\u6280\u672f\u76f8\u7ed3\u5408\uff0c\u7531\u56fd\u5bb6\u6765\u5b8c\u6210\u3002
1.1.7 \u5e38\u538b\u5ba4\u6e29\u7b49\u79bb\u5b50\u4f53\u8bf1\u53d8\u80b2\u79cd
\u5e38\u538b\u4f4e\u6e29\u7b49\u79bb\u5b50\u4f53\uff08Atmospheric and Room Temperature Plasma\uff09\u7b80\u79f0\u4e3aARTP\uff0c\u6307\u80fd\u591f\u5728\u5927\u6c14\u538b\u4e0b\u4ea7\u751f\u6e29\u5ea6\u572825-40 \u00b0C\u4e4b\u95f4\u7684\u3001\u5177\u6709\u9ad8\u6d3b\u6027\u7c92\u5b50\uff08\u5305\u62ec\u5904\u4e8e\u6fc0\u53d1\u6001\u7684\u6c26\u539f\u5b50\u3001\u6c27\u539f\u5b50\u3001\u6c2e\u539f\u5b50\u3001OH\u81ea\u7531\u57fa\u7b49\uff09\u6d53\u5ea6\u7684\u7b49\u79bb\u5b50\u4f53\u5c04\u6d41\u3002ARTP\u6280\u672f\u4f5c\u4e3a\u4e00\u79cd\u65b0\u578b\u7684\u7269\u7406\u65b9\u6cd5\uff0c\u5728\u5fae\u751f\u7269\u8bf1\u53d8\u80b2\u79cd\u9886\u57df\u6709\u7740\u5e7f\u9614\u7684\u5e94\u7528\u524d\u666f\u3002
\u7b49\u79bb\u5b50\u4f53\u4e2d\u9002\u5f53\u5242\u91cf\u7684\u6d3b\u6027\u7c92\u5b50\u4f5c\u7528\u4e8e\u5fae\u751f\u7269\uff0c\u80fd\u591f\u4f7f\u5fae\u751f\u7269\u7ec6\u80de\u58c1/\u819c\u7684\u7ed3\u6784\u53ca\u901a\u900f\u6027\u6539\u53d8\uff0c\u5e76\u5f15\u8d77\u57fa\u56e0\u635f\u4f24\uff0c\u83cc\u682a\u51fa\u73b0\u9057\u4f20\u7269\u8d28\u635f\u4f24\u540e\uff0c\u5fae\u751f\u7269\u542f\u52a8SOS\u4fee\u590d\u673a\u5236\uff0c\u5176\u8bf1\u5bfc\u4ea7\u751fDNA\u805a\u5408\u9176\u2163\u548cV\uff0c\u5b83\u4eec\u4e0d\u5177\u67093\u02ca\u6838\u9178\u5916\u5207\u9176\u6821\u6b63\u529f\u80fd\uff0c\u4e8e\u662f\u5728DNA\u94fe\u7684\u635f\u4f24\u90e8\u4f4d\u5373\u4f7f\u51fa\u73b0\u4e0d\u914d\u5bf9\u78b1\u57fa\uff0c\u590d\u5236\u4ecd\u80fd\u7ee7\u7eed\u524d\u8fdb\u3002\u5728\u6b64\u60c5\u51b5\u4e0b\u5141\u8bb8\u9519\u914d\u53ef\u589e\u52a0\u5b58\u6d3b\u7684\u673a\u4f1a\u3002ARTP\u5bf9\u9057\u4f20\u7269\u8d28\u9020\u6210\u7684\u635f\u4f24\uff0c\u591a\u6837\u6027\u8f83\u9ad8\uff1b\u53c8SOS\u8bf1\u5bfc\u4fee\u590d\u672c\u8eab\u4e3a\u5bb9\u9519\u6027\u4fee\u590d\uff0c\u56e0\u6b64\uff0cARTP\u591a\u6837\u6027\u7684\u635f\u4f24\u5c06\u53ef\u80fd\u5728\u4fee\u590d\u8fc7\u7a0b\u4e2d\u5305\u5bb9\u4e8eDNA\u94fe\u4e2d\uff0c\u5728\u5fae\u751f\u7269\u8fdb\u884c\u590d\u5236\u4fee\u590d\u65f6\uff0c\u5176\u53ef\u80fd\u5e26\u6765\u591a\u6837\u6027\u7684\u9519\u914d\u53ef\u80fd\u3002
ARTP\u5e94\u7528\u4e8e\u5fae\u751f\u7269\u7a81\u53d8\u80b2\u79cd\uff0c\u6210\u672c\u4f4e\u3001\u64cd\u4f5c\u65b9\u4fbf\uff0c\u6ca1\u6709\u5f88\u591a\u7269\u7406\u8bf1\u53d8\u8bbe\u5907\uff08\u5982\u79bb\u5b50\u675f\u6ce8\u5165\u7b49\uff09\u6240\u9700\u7684\u79bb\u5b50\u6216\u7535\u5b50\u52a0\u901f\u3001\u771f\u7a7a\u548c\u5236\u51b7\u7b49\u9644\u5c5e\u8bbe\u5907\uff1bARTP\u5bf9\u9057\u4f20\u7269\u8d28\u7684\u635f\u4f24\u673a\u5236\u591a\u6837\uff0c\u5177\u6709\u8f83\u9ad8\u7684\u6b63\u7a81\u53d8\u7387\uff0c\u7a81\u53d8\u6027\u80fd\u591a\u6837\uff0c\u5bf9\u4e8e\u771f\u83cc\u3001\u7ec6\u83cc\u3001\u85fb\u7c7b\u7b49\u90fd\u6709\u6548\u679c\uff1bARTP\u5bf9\u73af\u5883\u65e0\u6c61\u67d3\uff0c\u4fdd\u8bc1\u64cd\u4f5c\u8005\u7684\u4eba\u8eab\u5b89\u5168\uff0c\u65e0\u8bba\u7528\u4f55\u79cd\u6c14\u4f53\u653e\u7535\uff0c\u5176\u5747\u65e0\u6709\u5bb3\u6c14\u4f53\u4ea7\u751f\u3002[1]
5\u5316\u5b66\u8bf1\u53d8
\u7f16\u8f91
2.1.1 \u70f7\u5316\u5242
\u70f7\u5316\u5242\u80fd\u4e0e\u4e00\u4e2a\u6216\u51e0\u4e2a\u6838\u9178\u78b1\u57fa\u53cd\u5e94\uff0c\u5f15\u8d77DNA \u590d\u5236\u65f6\u78b1\u57fa\u914d\u5bf9\u7684\u8f6c\u6362\u800c\u53d1\u751f\u9057\u4f20\u53d8\u5f02\uff0c\u5e38\u7528\u7684\u70f7\u5316\u5242\u6709\u7532\u57fa\u78fa\u9178\u4e59\u916f\u3001\u4e9a\u785d\u57fa\u80cd\u3001\u4e59\u70ef\u4e9a\u80fa\u3001\u786b\u9178\u4e8c\u4e59\u916f\u7b49\u3002
\u7532\u57fa\u78fa\u9178\u4e59\u916f\uff08ethylmethane sulphonate,EMS) \u662f\u6700\u5e38\u7528\u7684\u70f7\u5316\u5242\uff0c\u8bf1\u53d8\u7387\u5f88\u9ad8\u3002\u5b83\u8bf1\u5bfc\u7684\u7a81\u53d8\u682a\u5927\u591a\u6570\u662f\u70b9\u7a81\u53d8\uff0c\u8be5\u7269\u8d28\u5177\u6709\u5f3a\u70c8\u81f4\u764c\u6027\u548c\u6325\u53d1\u6027\uff0c\u53ef\u75285%\u786b\u4ee3\u786b\u9178\u94a0\u4f5c\u4e3a\u7ec8\u6b62\u5242\u548c\u89e3\u6bd2\u5242\u3002
N- \u7532\u57fa- N'- \u785d\u57fa- N- \u4e9a\u785d\u57fa\u80cd\uff08NTG) \u662f\u4e00\u79cd\u8d85\u8bf1\u53d8\u5242\uff0c\u5e94\u7528\u5e7f\u6cdb\uff0c\u4f46\u6709\u4e00\u5b9a\u6bd2\u6027\uff0c\u64cd\u4f5c\u65f6\u5e94\u8be5\u6ce8\u610f\u3002\u5728\u78b1\u6027\u6761\u4ef6\u4e0b\uff0cNTG \u4f1a\u5f62\u6210\u91cd\u6c2e\u7532\u70f7\uff08CH2N2\uff09\uff0c\u5b83\u662f\u5f15\u8d77\u81f4\u6b7b\u548c\u7a81\u53d8\u7684\u4e3b\u8981\u539f\u56e0\u3002\u5b83\u7684\u6548\u5e94\u5f88\u53ef\u80fd\u662fCH2N2 \u5bf9DNA \u7684\u70f7\u5316\u4f5c\u7528\u5f15\u8d77\u7684[2]\u3002
\u786b\u9178\u4e8c\u4e59\u916f\uff08DMS) \u4e5f\u5f88\u5e38\u7528\uff0c\u4f46\u7531\u4e8e\u6bd2\u6027\u592a\u5f3a\uff0c\u76ee\u524d\u5f88\u5c11\u4f7f\u7528\u3002\u4e59\u70ef\u4e9a\u80fa\uff0c\u751f\u4ea7\u7684\u8f83\u5c11\uff0c\u5f88\u96be\u4e70\u5230\u3002\u4f7f\u7528\u6d53\u5ea60.0001%~0.1%\uff0c\u9ad8\u5ea6\u81f4\u764c\u6027\uff0c\u4f7f\u7528\u65f6\u9700\u8981\u4f7f\u7528\u7f13\u51b2\u6db2\u914d\u7f6e\u3002
2.1.2 \u78b1\u57fa\u7c7b\u4f3c\u7269
\u78b1\u57fa\u7c7b\u4f3c\u7269\u5206\u5b50\u7ed3\u6784\u7c7b\u4f3c\u5929\u7136\u78b1\u57fa\uff0c\u53ef\u4ee5\u63ba\u5165\u5230DNA \u5206\u5b50\u4e2d\u5bfc\u81f4DNA \u590d\u5236\u65f6\u4ea7\u751f\u9519\u914d\uff0cmRNA \u8f6c\u5f55\u7d0a\u4e71\uff0c\u529f\u80fd\u86cb\u767d\u91cd\u7ec4\uff0c\u8868\u578b\u6539\u53d8\u3002\u8be5\u7c7b\u7269\u8d28\u6bd2\u6027\u76f8\u5bf9\u8f83\u5c0f\uff0c\u4f46\u8d1f\u8bf1\u53d8\u7387\u5f88\u9ad8\uff0c\u5f80\u5f80\u4e0d\u6613\u5f97\u5230\u597d\u7684\u7a81\u53d8\u4f53\u3002\u4e3b\u8981\u67095- \u6c1f\u5c3f\u5627\u5576\uff085- FU) \u30015- \u6eb4\u5c3f\u5627\u5576\uff085- BU) \u30016- \u6c2f\u560c\u5464\u7b49\u3002\u7a0b\u4e16\u6e05\u7b49[25]\u75285- BU \u5bf9\u4ea7\u8272\u7d20\u83cc\uff08\u5206\u679d\u6746\u83ccT17- 2- 39\uff09 \u7ec6\u80de\u8fdb\u884c\u8bf1\u53d8\uff0c\u751f\u7269\u91cf\u5e73\u5747\u63d0\u9ad822.5%.
2\uff0e1.3 \u65e0\u673a\u5316\u5408\u7269
\u8bf1\u53d8\u6548\u679c\u4e00\u822c\uff0c\u5371\u9669\u6027\u8f83\u5c0f\u3002\u5e38\u7528\u7684\u6709\u6c2f\u5316\u9502\uff0c\u767d\u8272\u7ed3\u6676\uff0c\u4f7f\u7528\u65f6\u914d\u62100.1%~0.5%\u7684\u6eb6\u6db2\uff0c\u6216\u8005\u53ef\u4ee5\u76f4\u63a5\u52a0\u5230\u8bf1\u53d8\u56fa\u4f53\u57f9\u517b\u57fa\u4e2d\uff0c\u4f5c\u7528\u65f6\u95f4\u4e3a30min\uff5e2d\u3002\u4e9a\u785d\u9178\u6613\u5206\u89e3\uff0c\u6240\u4ee5\u73b0\u914d\u73b0\u7528\u3002\u5e38\u7528\u4e9a\u785d\u9178\u94a0\u548c\u76d0\u9178\u5236\u53d6\uff0c\u5c06\u4e9a\u785d\u9178\u94a0\u914d\u62100.01~0.1mol/L \u7684\u6d53\u5ea6\uff0c\u4f7f\u7528\u65f6\u52a0\u5165\u7b49\u6d53\u5ea6\u7b49\u4f53\u79ef\u7684\u76d0\u9178\u5373\u53ef\u3002
2.1.4 \u5176\u4ed6
\u76d0\u9178\u7f9f\u80fa\uff0c\u4e00\u79cd\u8fd8\u539f\u5242\uff0c\u4f5c\u7528\u4e8eC \u4e0a\uff0c\u4f7fG- C \u53d8\u4e3aA- T\u3002\u4e5f\u8f83\u5e38\u7528,\u4f7f\u7528\u6d53\u5ea6\u4e3a0.1%\uff5e0.5%\uff0c\u4f5c\u7528\u65f6\u95f460min\uff5e2h\u3002
\u6b64\u5916\uff0c\u8bf1\u53d8\u65f6\u5c06\u4e24\u79cd\u6216\u591a\u79cd\u8bf1\u53d8\u56e0\u5b50\u590d\u5408\u4f7f\u7528\uff0c\u6216\u8005\u91cd\u590d\u4f7f\u7528\u540c\u4e00\u79cd\u8bf1\u53d8\u56e0\u5b50\uff0c\u6548\u679c\u66f4\u4f73\u3002\u987e\u6b63\u534e\u7b49[7]\u4ee5\u8c37\u6c28\u9178\u68d2\u6746\u83ccATCC- 13761 \u4e3a\u51fa\u53d1\u83cc\u682a\uff0c\u7ecfDMS \u548cNTG \u591a\u6b21\u8bf1\u53d8\u5904\u7406\uff0c\u83b7\u5f97\u4e00\u682aL- \u7ec4\u6c28\u9178\u4ea7\u751f\u83cc\u3002
2\u3001\u8bf1\u53d8\u5242
2.1 \u8bf1\u53d8\u5242\u7684\u9009\u62e9
\u5728\u9009\u62e9\u8bf1\u53d8\u5242\u65f6\uff0c\u9700\u8981\u6ce8\u610f\u8bf1\u53d8\u5242\u7684\u4e13\u4e00\u6027\uff0c\u5373\u67d0\u4e00\u8bf1\u53d8\u5242\u6216\u8bf1\u53d8\u5904\u7406\u4f18\u5148\u4f7f\u57fa\u56e0\u7ec4\u7684\u67d0\u4e9b\u90e8\u5206\u53d1\u751f\u7a81\u53d8\u800c\u522b\u7684\u90e8\u5206\u5373\u4f7f\u6709\u4e5f\u5f88\u5c11\u53d1\u751f\u7a81\u53d8\u3002\u5bf9\u8bf1\u53d8\u5242\u4e13\u4e00\u6027\u7684\u5206\u5b50\u57fa\u7840\u4e0d\u5341\u5206\u4e86\u89e3\u4e07\u5c3d\u7ba1\u6709\u5173\u7684\u4fee\u590d\u9014\u5f84\u5fc5\u5b9a\u5bf9\u6b64\u6709\u5f71\u54cd\uff0c\u4f46\u5b83\u4eec\u7684\u5173\u7cfb\u5e76\u4e0d\u90a3\u4e48\u7b80\u5355\uff0c\u5176\u5b83\u5404\u79cd\u56e0\u7d20\uff0c\u5305\u62ec\u8bf1\u53d8\u5904\u7406\u7684\u73af\u5883\u6761\u4ef6\u4e5f\u80fd\u5f71\u54cd\u7a81\u53d8\u7c7b\u578b\u3002
\u5de5\u4e1a\u9057\u4f20\u5b66\u5bb6\u5f88\u96be\u6b63\u786e\u5730\u9884\u8a00\u6539\u826f\u67d0\u4e00\u83cc\u79cd\u65f6\u9700\u8981\u4f55\u79cd\u7c7b\u578b\u7684\u5206\u5b50\u6c34\u5e73\u7684\u7a81\u53d8\u3002\u56e0\u6b64\uff0c\u4e3a\u4e86\u4ea7\u751f\u7c7b\u578b\u5c3d\u53ef\u80fd\u591a\u7684\u7a81\u53d8\u4f53\uff0c\u6700\u9002\u5f53\u7684\u65b9\u6cd5\u662f\u91c7\u7528\u51e0\u79cd\u4e92\u8865\u7c7b\u578b\u7684\u8bf1\u53d8\u5904\u7406\u3002\u8fdc\u7d2b\u5916\u65e0\u7591\u662f\u6240\u6709\u8bf1\u53d8\u5242\u4e2d\u6700\u4e3a\u5408\u9002\u7684\uff0c\u4f3c\u4e4e\u53ef\u4ee5\u8bf1\u5bfc\u6240\u6709\u5df2\u77e5\u7684\u635f\u4f24\u7c7b\u578b\u3002\u91c7\u53d6\u6709\u6548\u3001\u5b89\u5168\u7684\u9884\u9632\u65b9\u6cd5\u4e5f\u5f88\u5bb9\u6613\u3002\u5728\u5316\u5b66\u8bf1\u53d8\u5242\u4e2d\uff0c\u6db2\u4f53\u8bd5\u5242\u6bd4\u7c89\u672b\u8bd5\u5242\u66f4\u6613\u8fdb\u884c\u5b89\u5168\u64cd\u4f5c\u3002\u7684\u53e6\u4e00\u4e2a\u4e0d\u5229\u56e0\u7d20\u662f\u5b83\u6709\u4ea7\u751f\u7d27\u5bc6\u8fde\u9501\u7684\u7a81\u53d8\u4e1b\u7684\u8d8b\u52bf\uff0c\u5c3d\u7ba1\u8fd9\u79cd\u6548\u5e94\u5728\u67d0\u4e9b\u4f53\u7cfb\u4e2d\u80fd\u6210\u4e3a\u6709\u5229\u6761\u4ef6\u3002\u6700\u540e\uff0c\u5fc5\u987b\u8ba4\u8bc6\u5230\u53ef\u80fd\u67d0\u4e9b\u7279\u5f02\u83cc\u7cfb\u7528\u67d0\u4e9b\u8bf1\u53d8\u5242\u662f\u4e0d\u80fd\u88ab\u8bf1\u53d8\u7684\u3002\u5f53\u7136\u8fd9\u4e00\u70b9\u901a\u8fc7\u6d4b\u5b9a\u6613\u68c0\u51fa\u7684\u7a81\u53d8\u4f53\uff0c\u5982\u6297\u836f\u6027\u7a81\u53d8\u4f53\u6216\u539f\u517b\u578b\u56de\u590d\u7a81\u53d8\u4f53\u7684\u8bf1\u53d8\u52a8\u529b\u5b66\u53ef\u4ee5\u76f8\u5f53\u5bb9\u6613\u5730\u5f97\u5230\u9a8c\u8bc1\u3002[8]
2.2 \u8bf1\u53d8\u5242\u7684\u5242\u91cf
\u4ece\u968f\u673a\u7b5b\u9009\u7684\u6700\u4f73\u6548\u679c\u770b\uff0c\u8bf1\u53d8\u5242\u7684\u6700\u9002\u5242\u91cf\u5c31\u662f\u5728\u7528\u4e8e\u7b5b\u9009\u7684\u5b58\u6d3b\u7fa4\u4f53\u4e2d\u5f97\u5230\u6700\u9ad8\u6bd4\u4f8b\u7684\u6240\u9700\u8981\u7684\u7a81\u53d8\u4f53\uff0c\u56e0\u4e3a\u8fd9\u4f1a\u4f7f\u5728\u6d4b\u5b9a\u6548\u4ef7\u7684\u9636\u6bb5\u66f4\u7701\u529b\u3002
\u56e0\u6b64\u5728\u83cc\u682a\u6539\u826f\u4ee5\u524d\uff0c\u4e3a\u4e86\u51b3\u5b9a\u6240\u7528\u8bf1\u53d8\u5242\u7684\u6700\u9002\u5242\u91cf\uff0c\u5e76\u4e3a\u7a81\u53d8\u6027\u7684\u589e\u5f3a\u6280\u672f\u6253\u4e0b\u57fa\u7840\uff0c\u806a\u660e\u7684\u505a\u6cd5\u901a\u5e38\u662f\u6d4b\u5b9a\u4e0d\u540c\u8bf1\u53d8\u5242\u5904\u7406\u4e0d\u540c\u83cc\u79cd\u65f6\u7684\u7a81\u53d8\u52a8\u529b\u5b66\u3002\u7528\u9ad8\u5355\u4f4d\u7a81\u53d8\u672c\u8eab\u6765\u6d4b\u5b9a\u6700\u9002\u5242\u91cf\u6709\u65f6\u662f\u4e0d\u53ef\u80fd\u7684\uff0c\u56e0\u4e3a\u8fd9\u79cd\u7a81\u53d8\u7684\u68c0\u6d4b\u5f88\u56f0\u96be\u3002\u4f46\u5982\u4f7f\u7528\u5bb9\u6613\u68c0\u51fa\u7684\u6807\u8bb0\u5982\u8010\u836f\u6807\u8bb0\uff0c\u53ea\u8981\u4f30\u8ba1\u5230\u65b9\u6cd5\u7684\u5c40\u9650\u6027\uff0c\u8fd8\u662f\u53ef\u4ee5\u63d0\u4f9b\u4e00\u4e9b\u6709\u4ef7\u503c\u7684\u8d44\u6599\u7684\u3002
对自然界中的微生物，在未经人工诱变或杂交处理的情况下进行分离和纯化（见微生物的分离和纯化），然后进行纯培养和测定（见微生物测定法），择优选取微生物的菌种。这种方法简单易行，但获得优良菌种的几率小，一般难以满足生产的需要。
绛旓細涔熺О鑷劧鍒嗙锛屾槸鎸囧寰敓鐗缁嗚優缇や綋涓嶇粡杩囦汉宸ュ鐞嗚岀洿鎺ヨ繘琛岀瓫閫夌殑鑲茬鏂规硶锛屽張绉颁负鍗曡弻钀藉垎绂汇鑷劧閫夎偛鐨勪富瑕佷綔鐢ㄦ槸瀵硅弻绉嶇函鍖栦互鑾峰緱閬椾紶鑳屾櫙杈冧负鍧囦竴鐨勭粏鑳炵兢浣撱備竴鑸弻绉嶇粡杩囧娆′紶浠ｆ垨闀挎湡淇濊棌鍚庯紝鐢变簬鑷劧绐佸彉鎴栧紓鏍镐綋鍜屽鍊嶄綋鐨勫垎绂伙紝浣挎湁浜涚粏鑳炵殑閬椾紶鎬х姸鍙戠敓鏀瑰彉锛岄犳垚鑿岀涓嶇函锛屼弗閲嶈呯敓浜ц兘鍔涗笅闄...
绛旓細瀵鑷劧鐣屼腑鐨寰敓鐗锛屽湪鏈粡浜哄伐璇卞彉鎴栨潅浜ゅ鐞嗙殑鎯呭喌涓嬭繘琛屽垎绂诲拰绾寲锛堣寰敓鐗╃殑鍒嗙鍜岀函鍖栵級锛岀劧鍚庤繘琛岀函鍩瑰吇鍜屾祴瀹氾紙瑙佸井鐢熺墿娴嬪畾娉曪級锛屾嫨浼橀夊彇寰敓鐗╃殑鑿岀銆傝繖绉嶆柟娉曠畝鍗曟槗琛岋紝浣嗚幏寰椾紭鑹弻绉嶇殑鍑犵巼灏忥紝涓鑸毦浠ユ弧瓒崇敓浜х殑闇瑕併
绛旓細1銆鑷劧閫夎偛 锛1锛夋病鏈夌粡杩囦汉宸ュ鐞嗭紝鍒╃敤鑿岀鐨勮嚜鐒绐佸彉骞剁瓫閫夊嚭鐞嗘兂鑿岀鐨勮繃绋嬩究鏄嚜鐒堕夎偛銆傦紙2锛夊湪娌℃湁浜哄伐骞查涓嬬殑鎯呭喌涓嬶紝閮ㄥ垎寰敓鐗鎵鍙戠敓鐨勭獊鍙樹究鏄嚜鐒剁獊鍙橈紝閫氬父灏嗚嚜鐒剁獊鍙樺垎涓烘绐佸彉鍜岃礋绐佸彉锛屽叾涓浜庣敓浜ф湁鐩婄殑绐佸彉渚挎槸姝ｇ獊鍙橈紝姣斿鑿屾牚鐢熶骇鎬ц兘鎻愬崌浜嗭紝鑰屼笉甯屾湜鍦ㄧ敓浜т笂鍙戠敓鐨勭獊鍙樹究鏄礋...
绛旓細1. 鑷劧閫夎偛锛氳嚜鐒堕夎偛鏄寚涓嶇粡杩囦汉宸ュ共棰勶紝鍒╃敤寰敓鐗╄嚜鐒剁獊鍙樼殑杩囩▼鏉ョ瓫閫夌悊鎯宠弻鏍殑鏂规硶銆傝繖绉嶆柟娉曚緷璧栦簬鑷劧鏉′欢涓寰敓鐗╃殑鍙樺紓锛屽垎涓烘湁鐩婄殑姝ｉ潰鍙樺紓鍜屾湁瀹崇殑璐熼潰鍙樺紓銆傛闈㈠彉寮傚彲浠ユ彁鍗囪弻鏍殑鐢熶骇鎬ц兘锛岃岃礋闈㈠彉寮傚彲鑳藉鑷磋弻鏍“閫鎴栫敓浜ц川閲忎笅闄嶃2. 璇卞彉鑲茬锛氳鍙樿偛绉嶆槸閫氳繃浣跨敤鐗╃悊鎴栧寲瀛﹁鍙樺墏鏉ユ彁...
绛旓細璇卞彉鑲茬 璇卞彉鑲茬鏄寚鐢ㄤ汉宸ョ殑鏂规硶澶勭悊寰敓鐗,浣垮畠浠彂鐢熺獊鍙,鍐嶄粠涓瓫閫夊嚭绗﹀悎瑕佹眰鐨勭獊鍙樿弻鏍,渚涚敓浜у拰绉戝瀹為獙鐢.璇卞彉鑲茬涓庡叾浠栬偛绉嶆柟娉曠浉姣,鍏锋湁鎿嶄綔绠渚裤侀熷害蹇拰鏀舵晥澶х殑浼樼偣,鑷充粖浠嶆槸涓绉嶉噸瑕佺殑銆佸箍娉涘簲鐢ㄧ殑寰敓鐗╄偛绉鏂规硶.璇卞彉鑲茬鍖呮嫭鍑哄彂鑿岀閫夋嫨銆佽鍙樺鐞嗗拰绛涢夌獊鍙樻牚涓変釜閮ㄥ垎.鑿岀缁忚鍙樺鐞...
绛旓細鑷劧閫夎偛锛堜粠鑷劧鐣岀洿鎺ュ垎绂荤函鍖栵級绠鍗曟槗琛岋紝浣嗚幏寰椾紭鑹弻绉嶅嚑鐜囧皬銆備汉宸ヨ偛绉嶄腑锛氳鍙樿偛绉嶅疄闄呬笂璺鑷劧鑲茬宸笉澶氾紝灏辨槸鐢ㄨ鍙戠獊鍙樼殑鏂瑰紡澧炲姞浜嗙獊鍙樼巼锛岀獊鍙樼巼楂樹絾鏈夌敤绐佸彉鐨勫嚑鐜囦綆锛岀浉褰撲簬鑷劧鑲茬鐣ュ井鍙戝睍涓涓嬶紝绋嶅井楹荤儲涓鐐癸紙鍏跺疄鏄搷浣滀汉鍛樺嵄闄╁ぇ锛夈傛潅浜よ偛绉嶉夯鐑﹀浜嗭紝鎿嶄綔鐜鍜屾妧鏈姹傝緝楂橈紝浣嗗彲闈...
绛旓細鑷劧閫夎偛鐨勮弻绉嶆潵婧愪簬鑷劧鐣屻佽弻绉嶄繚钘忔満鏋勬垨鐢熶骇杩囩▼,浠庤嚜鐒剁晫涓夎偛鑿岀鐨勮繃绋嬭緝涓哄鏉,鑰屼粠鐢熶骇杩囩▼鎴栬弻绉嶄繚钘忔満鏋勫緱鍒拌弻绉鐨勮嚜鐒堕夎偛杩囩▼杈冧负绠鍗曘傝嚜鐒堕夎偛鐨勬楠や富瑕佹槸:閲囨牱,澧為暱鍩瑰吇,鍩瑰吇鍒嗙鍜岀瓫閫夌瓑銆傞噰鏍 绛涢夌殑鑿岀閲囬泦鐨勫璞′互鍦熷￥涓轰富,涔熷彲浠ユ槸妞嶇墿銆佽厫璐ョ墿鍝佸拰鏌愪簺姘村煙绛夈傚湡澹ゆ槸寰敓鐗鐨勬眹闆嗗湴,浠庡湡澹や腑...
绛旓細1锛庤嚜鐒堕夎偛 闅忕潃寰敓鐗╁鐨勫彂灞曪紝鐗瑰埆鏄湪鍙戞槑寰敓鐗╃殑绾煿鍏绘妧鏈箣鍚庯紝鍑虹幇浜嗗井鐢熺墿绾鐨勮嚜鐒堕夎偛銆備互鍩哄洜鑷彂绐佸彉涓哄熀纭閫夎偛浼樿壇鎬х姸鑿屾牚鐨勮繖绉嶆柟娉曪紝鏄渶鏃╁簲鐢ㄥ井鐢熺墿閬椾紶瀛﹀師鐞嗭紟杩涜鑲茬瀹炶返鐨勪竴涓疄渚嬨傜敱浜庡井鐢熺墿浣撳唴瀛樺湪鍏夊娲汇佸垏琛ヤ慨澶嶃侀噸缁勪慨澶嶃佺揣鎬ュ懠鏁戜慨澶嶇瓑淇鏈哄埗浠ュ強DNA鑱氬悎閰剁殑鏍℃浣滅敤...
绛旓細缂栬緫 瀵鑷劧鐣屼腑鐨勫井鐢熺墿锛屽湪鏈粡浜哄伐璇卞彉鎴栨潅浜ゅ鐞嗙殑鎯呭喌涓嬭繘琛屽垎绂诲拰绾寲锛堣寰敓鐗╃殑鍒嗙鍜岀函鍖栵級锛岀劧鍚庤繘琛岀函鍩瑰吇鍜屾祴瀹氾紙瑙佸井鐢熺墿娴嬪畾娉曪級锛屾嫨浼橀夊彇寰敓鐗╃殑鑿岀銆傝繖绉嶆柟娉曠畝鍗曟槗琛岋紝浣嗚幏寰椾紭鑹弻绉嶇殑鍑犵巼灏忥紝涓鑸毦浠ユ弧瓒崇敓浜х殑闇瑕併3浜哄伐閫夎偛 缂栬緫 鍒嗚鍙鑲茬鍜屾潅浜よ偛绉嶄袱绉嶃傝鍙樿偛绉 浠ヨ鍙...
绛旓細鍙戦叺宸ョ▼鏄寚閲囩敤鐜颁唬宸ョ▼鎶鏈墜娈碉紝鍒╃敤寰敓鐗╃殑鏌愪簺鐗瑰畾鍔熻兘锛屼负浜虹被鐢熶骇鏈夌敤鐨勪骇鍝侊紝鎴栫洿鎺ユ妸寰敓鐗╁簲鐢ㄤ簬宸ヤ笟鐢熶骇杩囩▼鐨勪竴绉嶆妧鏈傚彂閰靛伐绋嬬殑鍐呭鍖呮嫭鑿岀閫夎偛銆佸煿鍏诲熀鐨勯厤缃佺伃鑿屻佺瀛愭墿澶у煿鍏诲拰鎺ョ銆佸彂閰佃繃绋嬪拰浜у搧鐨勫垎绂绘彁绾紙鐢熺墿鍒嗙宸ョ▼锛夌瓑鏂归潰銆備紭鑹弻鏍鐨勯夎偛鐨勭洰鐨勬槸闃叉鑿岀閫銆佽В鍐崇敓浜у疄闄...
