我养的肺癌A549细胞株，发现里面圆形的细胞越来越多，梭形的细胞越来越少，这是怎么回事 h1650细胞带有什么基因突变
A549,NCI-H1299,H1650\u5747\u4e3a\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7ec6\u80de\u682a,3\u8005\u6709\u4f55\u533a\u522bNCI-H1650 \u7ec6\u652f\u6c14\u7ba1\u80ba\u6ce1\u764c\uff0c\u80ba\u817a\u764c
NCI-H1299 \u80ba\u764c\uff0c\u8d77\u6e90\u4e8e\u795e\u7ecf\u5185\u5206\u6ccc
A549 \u80ba\u764c
\u5177\u4f53\u7684\u53bbATCC\u67e5\u8be2
\u8be5\u7ec6\u80de\u662f\u4ece\u4e00\u540d27\u5c81\u767d\u4eba\u7537\u6027\uff0810\u5e74\u70df\u9f84\uff09\u652f\u6c14\u7ba1\u80ba\u6ce1\u764c\u60a3\u8005\u7684\u80f8\u8154\u79ef\u6db2\u4e2d\u5206\u79bb\u5f97\u5230\u7684\u3002HCC827\u548cH1650\u4e3a\u540c\u79cd\u7a81\u53d8\u7c7b\u578b\uff0c\u4e3aEGFR E746-A750\u7f3a\u5931\u3002
要分裂的，正常表征。绛旓細浼氾紒鐧岀粏鑳炴湁鍙兘鍦ㄧ粍缁囬噷涓嶈鎺掓枼鈥︾劧鍚庣箒娈栤︾劧鍚庤浆绉烩︹﹀摝锛佸浜嗭紝閭ｆ槸绉戝够灏忚鐨勩傛病浜嬬殑锛屼笉鏄綘鐨勪綋缁嗚優锛灏辩畻鏄椿鐨勪篃浼氳骞叉帀鐨勩傛斁蹇冨惂銆備汉浣撶殑鍏嶇柅绯荤粺涓嶄細閭ｄ箞宸姴銆
绛旓細澶ч粍閰歌兘鏈夋晥鎶戝埗鑲虹檶缁嗚優A549鐨勭敓闀匡紝閫氳繃鍥涚敳鍩哄伓姘攽鐩愭瘮鑹叉硶(MTT娉曪級鍙戠幇澶ч粍閰告姂鍒A549缁嗚優鐨勭敓闀裤傚噵浜″疄楠屾樉绀哄ぇ榛勯吀澶勭悊鐨勭粏鑳炴槑鏄惧憟鐜板嚭鏌撹壊璐ㄨ竟缂樺寲锛屽嚭鐜板噵浜″皬浣擄紝鏍稿浐缂╃瓑鍑嬩骸褰㈡侊紝璇存槑澶ч粍閰歌兘寮曡捣鐧岀粏鑳炵殑鍑嬩骸銆傞氳繃閰嶄綋绔炰簤鎬у疄楠岋紝缁撴灉鍙戠幇澶ч粍閰稿彲浠ョ珵浜9-cis-RA鍜孯XR缁撳悎銆傚湪鏇磋缁嗙殑...
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绛旓細澶ч粍閰稿睍鐜板嚭寮哄ぇ鐨勬姉鐧岀壒鎬э紝鐗瑰埆閽堝鑲虹檶缁嗚優A549銆備娇鐢∕TT娉曪紙鍥涚敳鍩哄伓姘攽鐩愭瘮鑹叉硶锛夌殑鐮旂┒鎻ず锛屽ぇ榛勯吀鏄捐憲鎶戝埗浜嗚繖浜涚粏鑳炵殑鐢熼暱銆傚噵浜″疄楠屾樉绀猴紝澶ч粍閰稿鐞嗗悗鐨勭粏鑳鍛堢幇鍑烘樉钁楃殑鍑嬩骸鐗瑰緛锛屽鏌撹壊璐ㄨ竟缂樺寲銆佸舰鎴愬噵浜″皬浣撳拰鏍稿浐缂╋紝璇佸疄浜嗗畠瀵圭檶缁嗚優鍑嬩骸鐨勮瀵间綔鐢ㄣ傝繘涓姝ョ殑閰嶄綋绔炰簤鎬у疄楠鍙戠幇锛澶ч粍閰...
绛旓細瀵硅繖浜涘ぇ榧鐨勮偤鐏屾礂娑插苟瀵硅偤缁勭粐鐥呯悊鍒囩墖鍒嗘瀽鍚鍙戠幇,PM2.5鑳藉寮曡捣鑲洪儴琛绠￠氶忔х殑鏀瑰彉銆佽偤缁嗚優鎹熶激鍜屽姞閲嶆哀鍖栧簲婵鎹熶激,鍦ㄩ珮鍓傞噺鏌撴瘨缁,澶ч紶鑲洪儴鐐庢х粏鑳炴笚鍑,鑲洪棿闅旀按鑲裤 2009骞寸殑涓椤瑰疄楠岄噰闆嗕簡鍖椾含鍩庡尯澶ф皵涓殑PM2.5,浠ヤ汉鑲烘场涓婄毊缁嗚優鏍(A549)涓烘ā鍨嬭繘琛屾瘨鐞嗕綔鐢ㄧ爺绌躲傚湪杩欎釜瀹為獙涓,浠25銆50銆100銆200渭g/ml...
绛旓細GPs鍦ㄤ綋澶1-10渭g/ml鏃跺鑲虹檶缁嗚優鏍狝549銆丆alu1銆592/9鐨勬姂鍒朵綔鐢ㄦ槑鏄惧己浜庡棰堢檶Hela S3鍙婄粨鑲犺吅鐧孋olo205,鑰屽悓鏍锋祿搴Ps瀵规甯镐汉娣峰悎娣嬪反缁嗚優涓嶄絾鏃犳姂鍒朵綔鐢,鍗存湁淇冭繘缁嗚優澧炴畺鐨勪綔鐢ㄣ傜粸鑲¤摑鎻愬彇娑层1.0g(鐢熻嵂)/ml鍚殏鐢6.44mg/ml,澶氱硸10.697mg/ml銆曞湪1-20渭l/ml鏃,瀵逛汉澶ц偁鐧岀粏鑳(Hce8693)鐨凞NA鍚堟垚鏈夋姂鍒...
绛旓細涔熷彲浠ュ彇寰椾笉閿欑殑娌荤枟鏁堟灉銆傛湁鐮旂┒璇佹槑锛氫腑鍗楀ぇ瀛︽箻闆呭尰闄㈣儭纭曠瓑妫娴嬩簡 浜哄弬鐨傝嫹 Rh2瀵 鑲鸿吅鐧 A549缁嗚優 銆侀『閾 鑰愯嵂 缁嗚優A549 DDP鐨勬姂鍒剁巼銆鍙戠幇浜哄弬鐨傝嫹Rh2瀵硅繖涓ょ缁嗚優鍧囨湁鏄庢樉鎶戝埗浣滅敤锛屼笖闅忕潃 娴撳害 鐨勫鍔狅紝浜哄弬鐨傝嫹Rh2瀵笰549 DDP缁嗚優鐨勬姂鍒剁巼鏄庢樉澧炲己銆傝〃鏄庝汉鍙傜殏鑻稲h2鑳戒績浣緼549 DDP缁嗚優鍑嬩骸銆
绛旓細1.鐮旂┒鐘榛勪父瀵逛汉涔宠吅鐧岀粏鑳炴牚MDA-MB-231銆佷汉鑲濈檶缁嗚優鏍猄MMC7721銆佷汉鑶鑳辩檶缁嗚優鏍猅24銆佷汉鏃╁辜绮掔粏鑳炵櫧琛鐥呯粏鑳炴牚HL-60銆佷汉鑲鸿吅鐧孉549缁嗚優鏍缁嗚優澧炴畺鐨勫奖鍝 鏂规硶:灏嗕笉鍚屾祿搴︾妧榛勪父娴稿嚭娑,鐩存帴鍔犲叆鍚偪鐦ょ粏鑳炲煿鍏绘澘涓,MTT娉曞垎鍒祴瀹氬叾瀵瑰绉嶆伓鎬ц偪鐦ょ粏鑳炴牚澧炴畺鐨勫奖鍝嶃傜粨鏋:鐘榛勪父娴稿嚭娑插MDA-MB-231...
绛旓細缁嗚優绯 缁嗚優绫诲瀷 绉 缁勭粐 鎺ㄨ崘浣跨敤鐨勫煿鍏诲熀 293 鎴愮氦缁寸粏鑳 浜 鑳氳儙鑲 MEM,10% 鐑伃娲婚┈琛娓 3T6 鎴愮氦缁寸粏鑳 灏忛紶 鑳氳儙 DMEM,10% 鑳庣墰琛娓 A549 涓婄毊缁嗚優 浜 鑲虹檶 F-12K,10%鑳庣墰琛娓 A9 鎴愮氦缁寸粏鑳 灏忛紶 缁撶紨缁勭粐 DMEM,10%鑳庣墰琛娓 AtT-20 涓婄毊缁嗚優 灏忛紶 鍨備綋鑲跨槫 F-10,15% 椹娓呭拰...
绛旓細A549鏄鑲虹檶缁嗚優绯
