血清代谢组学研究、 什么是医学?
\u4ec0\u4e48\u662f\u533b\u5b66\u533b\u5b66\uff0c\u662f\u7528\u6551\u6b7b\u6276\u4f24\uff0c\u60ac\u58f6\u6d4e\u4e16\u7684\u5fc3\u6765\u4e3a\u4eba\u7c7b\u670d\u52a1\u7684\uff0c\u6ca1\u6709\u6b64\u5fc3\uff0c\u4e0d\u914d\u4e3a\u533b\uff01
\u800c\u8fd9\u4e2a\u533b\uff0c\u5c31\u662f\u6307\u533b\u6cbb\u3001\u533b\u7597\u3001\u533b\u836f\u3002
\u7b3c\u7edf\u7684\u8bb2\uff0c\u533b\u5b66\u5c31\u662f\u6307\u7528\u4e00\u5b9a\u7684\u6cbb\u7597\u624b\u6bb5\uff0c\u7597\u6548\u65b9\u6cd5\uff0c\u4e34\u5e8a\u836f\u7269\uff0c\u4e3a\u5e7f\u5927\u75c5\u60a3\u8005\u63d0\u4f9b\u670d\u52a1\u7684\u4e00\u4e2a\u5b66\u79d1\u3002\u8fd9\u4e5f\u53ea\u662f\u72ed\u4e49\u7684\u5b9a\u4e49\uff01
\u5e7f\u4e49\u7684\u8bb2\uff0c\u533b\u5b66\u5c31\u662f\u4ee5\u4eba\u4e3a\u670d\u52a1\u5bf9\u8c61\uff0c\u901a\u8fc7\u793e\u4f1a-\u751f\u7269-\u4e2a\u4eba\u6a21\u5f0f\uff0c\u7528\u4ee5\u4e00\u5207\u7684\u533b\u7597\u65b9\u6cd5\u4e3a\u4eba\u7c7b\u670d\u52a1\u7684\u4e00\u95e8\u5b66\u79d1\u3002
\u8fd9\u5c31\u7a81\u51fa\u4e86\u5bf9\u201c\u4eba\u201d\u7684\u670d\u52a1\u3002\u4f5c\u4e3a\u533b\u751f\uff0c\u773c\u91cc\u4e0d\u80fd\u53ea\u6709\u201c\u75c5\u201d\uff0c\u6ca1\u6709\u201c\u4eba\u201d\u3002
\u4f46\u613f\u6211\u7684\u56de\u7b54\u80fd\u5bf9\u4f60\u6709\u6240\u5e2e\u52a9\uff01
\u533b\u5b66\uff0c\u662f\u901a\u8fc7\u79d1\u5b66\u6216\u6280\u672f\u7684\u624b\u6bb5\u5904\u7406\u751f\u547d\u7684\u5404\u79cd\u75be\u75c5\u6216\u75c5\u53d8\u7684\u4e00\u79cd\u5b66\u79d1\uff0c\u4fc3\u8fdb\u75c5\u60a3\u6062\u590d\u5065\u5eb7\u7684\u4e00\u79cd\u4e13\u4e1a\u3002\u5b83\u662f\u751f\u7269\u5b66\u7684\u5e94\u7528\u5b66\u79d1\uff0c\u5206\u57fa\u7840\u533b\u5b66\u3001\u4e34\u5e8a\u533b\u5b66\u3002\u4ece\u751f\u7406\u89e3\u5256\u3001\u5206\u5b50\u9057\u4f20\u3001\u751f\u5316\u7269\u7406\u7b49\u5c42\u9762\u6765\u5904\u7406\u4eba\u4f53\u75be\u75c5\u7684\u9ad8\u7ea7\u79d1\u5b66\u3002\u5b83\u662f\u4e00\u4e2a\u4ece\u9884\u9632\u5230\u6cbb\u7597\u75be\u75c5\u7684\u7cfb\u7edf\u5b66\u79d1\uff0c\u7814\u7a76\u9886\u57df\u5927\u65b9\u5411\u5305\u62ec\u57fa\u7840\u533b\u5b66\u3001\u4e34\u5e8a\u533b\u5b66\u3001\u6cd5\u533b\u5b66\u3001\u68c0\u9a8c\u533b\u5b66\u3001\u9884\u9632\u533b\u5b66\u3001\u4fdd\u5065\u533b\u5b66\u3001\u5eb7\u590d\u533b\u5b66\u7b49\u3002
\u533b\u5b66\u53ef\u5206\u4e3a\u73b0\u4ee3\u533b\u5b66\uff08\u5373\u901a\u5e38\u8bf4\u7684\u897f\u533b\u5b66\uff09\u548c\u4f20\u7edf\u533b\u5b66\uff08\u5305\u62ec\u4e2d\uff08\u6c49\uff09\u533b\u3001\u85cf\u533b\u3001\u8499\u59ff\u65cf\u533b\u3001\u7ef4\u533b\u3001\u671d\u533b\u3001\u5f5d\u533b\u3001\u58ee\u533b\u3001\u82d7\u533b\u3001\u50a3\u533b\u7b49\uff09\u591a\u79cd\u533b\u5b66\u4f53\u7cfb\u3002\u4e0d\u540c\u5730\u533a\u548c\u6c11\u65cf\u90fd\u6709\u76f8\u5e94\u7684\u4e00\u4e9b\u533b\u5b66\u4f53\u7cfb\uff0c\u5b97\u65e8\u548c\u76ee\u7684\u4e0d\u76f8\u540c\u3002\u5370\u5ea6\u4f20\u7edf\u533b\u5b66\u7cfb\u7edf\u4e5f\u88ab\u8ba4\u4e3a\u5f88\u53d1\u8fbe\u3002
\u7814\u7a76\u9886\u57df\u5927\u65b9\u5411\u5305\u62ec\u57fa\u7840\u533b\u5b66\u3001\u4e34\u5e8a\u8ff9\u8fc5\u5f0a\u533b\u5b66\u3001\u6cd5\u533b\u5b66\u3001\u68c0\u9a8c\u533b\u5b66\u3001\u9884\u9632\u533b\u5b66\u3001\u4fdd\u5065\u533b\u5b66\u3001\u5eb7\u590d\u533b\u5b66\u7b49\u3002
\u57fa\u7840\u533b\u5b66\u5305\u62ec\uff1a\u533b\u5b66\u751f\u7269\u6570\u5b66\uff0c\u533b\u5b66\u751f\u7269\u5316\u5b66\uff0c\u533b\u5b66\u751f\u7269\u7269\u7406\u5b66\uff0c\u4eba\u4f53\u89e3\u5256\u5b66\uff0c\u533b\u5b66\u7ec6\u80de\u751f\u7269\u5b66\uff0c\u4eba\u4f53\u751f\u7406\u5b66\uff0c\u4eba\u4f53\u7ec4\u7ec7\u5b66\uff0c\u4eba\u4f53\u80da\u80ce\u5b66\uff0c\u533b\u5b66\u9057\u4f20\u5b66\uff0c\u4eba\u4f53\u514d\u75ab\u5b66\uff0c\u533b\u5b66\u5bc4\u751f\u866b\u5b66\uff0c\u533b\u5b66\u5fae\u751f\u7269\u5b66\uff0c\u533b\u5b66\u75c5\u6bd2\u5b66\uff0c\u4eba\u4f53\u75c5\u7406\u5b66\uff0c\u75c5\u7406\u751f\u7406\u5b66\uff0c\u836f\u7406\u5b66\uff0c\u533b\u5b66\u5b9e\u9a8c\u52a8\u7269\u5b66\uff0c\u533b\u5b66\u5fc3\u7406\u5b66\uff0c\u751f\u7269\u533b\u5b66\u5de5\u7a0b\u5b66\uff0c\u533b\u5b66\u4fe1\u606f\u5b66\uff0c\u6025\u6551\u660c\u95f7\u5b66\uff0c\u62a4\u75c5\u5b66\uff0c\u65b0\u4e2d\u5fc3\u6cd5\u5219
总的来说,动静脉血的差别不大,血清和血浆的代谢物差别也并没有特别明显,只要取样量一致,样品类型一致,样品不溶血污染,平行性好,都可以做代谢组学的分析。
不知道你要做什么代谢实验,如果是人和有血液的动物的代谢,用血清比较好,血浆中有血细胞,不容易分离
绛旓細鐞嗚鏉ヨ锛岃娓鍋浠h阿缁勫鏁堟灉浼氭洿濂斤紝琛娓呬腑浠h阿鐗╃殑鍚噺鐣ラ珮浜庤娴嗐傝吂涓诲姩鑴夊彇鍑虹殑琛杈冨锛屽洜姝よ娓呮垨琛娴嗘牱鍝佸潎鍙互浣滀负浠h阿缁勫鏍峰搧杩涜鍒嗘瀽銆傜溂鐪跺彇琛閲忓皯锛屽苟涓斿彇琛鎵嬫硶锛岀敤鍏凤紝鐜绛夊洜绱犲共鎵拌緝澶э紝鍒嗙琛娓呮椂甯稿父浼氬彂鐢熸憾琛鐨勬儏鍐碉紝鍥犳寤鸿浣跨敤鑲濈礌閽犳姉鍑濈鍙栬娴嗭紝浼氫娇鏍峰搧鐨勫钩琛屾ц緝濂姐傝嫢鏈...
绛旓細琛娓鍜岃娴嗘牱鏈兘琚箍娉涚敤浜浠h阿缁勫鐮旂┒锛鏁翠綋鑰岃█锛岃娓呭拰琛娴嗙殑浠h阿鐗╃绫诲樊寮備笉澶э紝浣嗘槸澶氭暟浠h阿鐗╁湪涓ょ鏍锋湰涓祿搴﹀樊寮傝緝澶с傚彧瑕佺‘淇濇牱鏈敹闆嗚繃绋嬬殑涓鑷存с傚悓涓涓」鐩娇鐢ㄧ殑鏍锋湰涓鑷达紙閮芥槸琛娴嗘垨閮芥槸琛娓咃級锛岀敱浜庤娓呮牱鏈渶瑕佹椂闂村幓鍑濈粨锛屽挨鍏惰娉ㄦ剰灏藉彲鑳戒繚璇佹墍鏈夋牱鍝佸嚌琛鏃堕棿鐨勪竴鑷存с傝娓呭拰琛娴嗘牱鏈...
绛旓細閫氬父閲囩敤闈欒剦鎶借锛屼娇鐢ㄦ棤鑿岄噰琛绠★紝鍙互閫夋嫨鍚湁鎴栦笉鍚湁鎶楀嚌鍓傜殑绠″瓙锛岃鍚庣画澶勭悊鍜屽垎鏋愰渶姹傝屽畾銆3.鏍锋湰閲忥細閫氬父闇瑕1-2 mL琛娑层備簩銆佽娑叉牱鏈鐞嗭細1.绂诲績锛氬皢琛娑叉牱鏈湪4掳C涓嬭繘琛岀蹇冿紙渚嬪锛3000 g锛10鍒嗛挓锛夛紝浠ュ垎绂昏娴嗘垨琛娓銆傝娴嗭細鎶借鍚庯紝绔嬪嵆鍦4掳C涓嬬蹇冿紙渚嬪锛3000 g锛10鍒嗛挓锛変互...
绛旓細甯傚満浠介锛14 鍏徃浜烘暟锛氱害300浜 鍏徃浠嬬粛锛氬浗鍐呮渶鏃╀笓闂ㄤ粠浜浠h阿缁勫绉戠爺鏈嶅姟鐨勫叕鍙镐箣涓锛屼富鎵撻潪闈舵爣浠h阿缁勫妫娴嬪垎鏋愭湇鍔★紝鍏朵紭鍔挎槸鏁版嵁璐ㄩ噺锛屾嵁璇磋兘鍋氬埌缇庡浗metabolon鐨勬按骞筹紝CV<10%銆傚叕鍙稿垱濮嬩汉婧愯嚜浜庡鏃﹀ぇ瀛﹀強涓闄紝棣栧腑绉戝椤鹃棶涓轰唬璋㈢粍瀛﹀浼氱涔﹂暱銆佷腑绉戦櫌鏈鐮旂┒鍛樸2.杩堢壒缁村皵 鎴愮珛鏃堕棿锛2015 鍦...
绛旓細鍥涘ぇ缁勫鏄寚鍩哄洜缁勫銆佽泲鐧借川缁勫銆浠h阿缁勫鍜岃浆褰曠粍瀛︺1銆佸熀鍥犵粍瀛︼細閫氳繃DNA鑺墖鎴栭珮閫氶噺娴嬪簭鎶鏈澶ц妯″熀鍥犵粍鏁版嵁杩涜鍒嗘瀽锛屼粠鑰屾壘鍑轰笌鐤剧梾鐩稿叧鐨勯仐浼犲彉寮傚強鍏跺熀鍥犺〃杈炬儏鍐点2銆佽泲鐧借川缁勫锛氶氳繃璐ㄨ氨銆佸厤鐤嵃杩圭瓑鎶鏈壌瀹氱柧鐥呯姸鎬佷笅涓嶅悓鏍锋湰锛堝琛娓銆佺粍缁囩瓑锛変腑铔嬬櫧璐ㄨ〃杈惧樊寮傦紝鎵惧嚭涓庣柧鐥呯浉鍏崇殑...
绛旓細鍦ㄨ泲鐧借川缁勫灞傞潰锛孲erpinA3N鐨勮〃杈惧湪SALD涓樉钁楀鍔狅紝鏄剧ず鍑轰笌鐐庣棁鐨勭揣瀵嗚仈绯汇侱EP鍒嗘瀽鏄剧ずSerpinA3N涓婅皟鐨勫悓鏃讹紝Mup1瀹舵棌鎴愬憳鍑忓皯锛岃繖浜涘彉鍖栧彲鑳藉奖鍝峉ALD鐨勫彂灞曡繘绋嬨浠h阿缁勫鐮旂┒鍒欏彂鐜帮紝CLP鍚庤娑蹭腑鐨勪唬璋㈤斿緞鍙戠敓鏄捐憲鍙樺寲锛屽叾涓22涓唬璋㈢墿鏄剧ず鍑洪珮搴︾殑宸紓鎬с傚缁村害瑙g爜锛歋erpinA3N涓庣値鐥囩殑绾藉甫</锛...
绛旓細缁у懆寮哄疄楠屽涔嬪悗锛岃タ婀栧ぇ瀛︾敓鍛界瀛﹀闄I閮ぉ鍗楀甫棰嗙殑铔嬬櫧璐ㄧ粍澶ф暟鎹疄楠屽锛岃繎鏃ュ湪鏂板啝鐥呮瘨鐮旂┒鏂归潰鍙堟湁閲嶈鍙戠幇銆備粬浠拰鍚堜綔鍥㈤槦涓璧峰鏂板啝鑲虹値鎮h呰娑蹭腑鐨勮泲鐧借川鍜浠h阿鐗╁垎瀛愯繘琛岀郴缁熸娴嬶紝鍙戠幇閲嶇棁鎮h呯殑琛娓涓瓨鍦ㄥ绉嶇嫭鐗圭殑鍒嗗瓙鍙樺寲锛屽苟鎵惧埌浜嗕竴绯诲垪鐢熺墿鏍囧織鐗╋紝鏈夋湜涓洪娴嬭交鐥囨偅鑰呭悜閲嶇棁鍙戝睍鎻愪緵瀵煎悜銆
绛旓細涓婃捣浜ら氬ぇ瀛﹁嵂瀛﹂櫌鏈夊叧涓撳閫氳繃浠h阿缁勫璇曢獙锛岀爺绌鍙戠幇浜嗘櫘娲辫尪闄嶈儐鍥洪唶銆侀檷琛鑴傜殑鑽鏈哄埗锛屼粠鑰屼负姝ゅ墠鍙檺浜庡吀绫嶈杞藉拰鑼跺弸浣撻獙鐨勮繖涓娉曟壘鍒颁簡鑽鏍规嵁銆傝繖涓爺绌剁敱涓婃捣浜ら氬ぇ瀛﹁嵂瀛﹂櫌鍓櫌闀胯淳浼熺瓑涓撳瀹屾垚锛屾槸涓婃捣浜ゅぇ涓庝簯鍗楄タ鍙岀増绾冲偅鏃忚嚜娌诲窞鏀垮簻鍚堜綔椤圭洰涔嬩竴銆傜爺绌舵垚鏋滃凡鍦ㄩ┈鏉ヨタ浜氫妇鍔炵殑涓涓浗闄呰尪鏂囧寲...
绛旓細涓嶇煡閬撲綘瑕佸仛浠涔堜唬璋㈠疄楠岋紝濡傛灉鏄汉鍜屾湁琛娑茬殑鍔ㄧ墿鐨浠h阿锛鐢琛娓姣旇緝濂斤紝琛娴嗕腑鏈夎缁嗚優锛屼笉瀹规槗鍒嗙
绛旓細璧典簯涓藉コ澹笓娉ㄤ簬鑽墿鐮旂┒锛濂圭殑瀛︽湳鍘嗙▼涓秹鍙婂涓噸瑕佽棰樸傞鍏堬紝濂瑰湪2011骞磋嚦2013骞存湡闂达紝鍙備笌浜嗙敱鍥藉鑷劧绉戝鍩洪噾鏀寔鐨勯」鐩紝鐮旂┒涓婚鏄椿琛鍖栫榾涓嵂澶嶆柟鐨勮嵂鏁堢墿璐ㄥ熀纭鍙婂叾閰嶄紞瑙勫緥锛岃繖琛ㄦ槑濂瑰湪浠h阿缁勫鍜琛娓鑽悊瀛﹂鍩熸湁鐫娣卞叆鐨勬帰绱傜揣鎺ョ潃锛屽ス鍦2010骞磋嚦2012骞达紝閫氳繃鏁欒偛閮ㄩ珮绛夊鏍″崥澹绉戠偣...