菌种培养基配方有哪些？ 液体菌种培养基配方有哪些？
\u4e00\u7ea7\u83cc\u79cd\u57f9\u517b\u57fa\u5e38\u7528\u7684\u914d\u65b9\u662f\u4ec0\u4e48\uff081\uff09\u4e00\u7ea7\u79cd\u57f9\u517b\u57fa\u914d\u65b9\u9a6c\u94c3\u85af\u8461\u8404\u7cd6\u743c\u8102\u57f9\u517b\u57fa\uff08PDA\u57f9\u517b\u57fa\uff09\uff1a\u53bb\u76ae\u9a6c\u94c3\u85af200\u514b\uff0c\u8461\u8404\u7cd620\u514b\uff0c\u743c\u810220\u514b\u3002\u6c341000\u6beb\u5347\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u9a6c\u94c3\u85af\u7efc\u5408\u57f9\u517b\u57fa\uff08CPDA\u57f9\u517b\u57fa\uff09\uff1a\u53bb\u76ae\u9a6c\u94c3\u85af200\u514b\uff0c\u8461\u8404\u7cd620\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be3\u514b\uff0c\u786b\u9178\u95411.5\u514b\uff0c\u7ef4\u751f\u7d20B10.05\u6beb\u514b\uff0c\u743c\u810220\u514b\uff0c\u6c341000\u6beb\u5347\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u9a6c\u94c3\u85af\u9175\u6bcd\u818f\u57f9\u517b\u57fa\uff08PDYA \u57f9\u517b\u57fa\uff09\uff1a\u53bb\u76ae\u9a6c\u94c3\u85af200\u514b\uff0c\u8461\u8404\u7cd620\u514b\uff0c\u9175\u6bcd\u818f1.5\u514b\uff0c\u743c\u810220\u514b\uff0c\u6c341000\u6beb\u5347\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u9ea6\u76ae\u8461\u8404\u7cd6\u57f9\u517b\u57fa\uff1a\u9ea6\u76ae50\uff5e70\u514b\uff0c\u8461\u8404\u7cd620\u514b\uff0c\u743c\u810220\u514b\uff0c\u6c341000\u6beb\u5347\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\uff082\uff09\u4e8c\u3001\u4e09\u7ea7\u79cd\u57f9\u517b\u57fa\u914d\u65b9\u7caa\u8349\u57f9\u517b\u57fa\uff1a\u53d1\u9175\u9ea6\u79c6\uff08\u7a3b\u8349\uff0972%\uff0c\u53d1\u9175\u725b\u7caa\u7c8920%\uff0c\u9ea6\u76ae5%\uff0c\u8517\u7cd61%\uff0c\u78b3\u9178\u94991%\uff0c\u542b\u6c34\u91cf62%\uff5e65%\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u725b\u7caa\u7c89\u57f9\u517b\u57fa\uff1a\u53d1\u9175\u725b\u7caa\u7c8998%\uff0c\u78b3\u9178\u94992%\uff0c\u542b\u6c34\u91cf62%\uff5e65%\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u68c9\u7c7d\u58f3\u57f9\u517b\u57fa\uff1a\u53d1\u9175\u68c9\u7c7d\u58f397%\uff0c\u8fc7\u78f7\u9178\u94991%\uff0c\u77f3\u70701%\uff0c\u78b3\u9178\u94991%\uff0c\u542b\u6c34\u91cf62%\uff5e65%\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u5c0f\u9ea6\u725b\u7caa\u7c89\u57f9\u517b\u57fa\uff1a\u5c0f\u9ea688%\uff0c\u725b\u7caa\u7c8910%\uff0c\u78b3\u9178\u94992%\uff0c\u542b\u6c34\u91cf\u9002\u5b9c\uff0c\u9178\u78b1\u5ea6\u81ea\u7136\u3002
\u5927\u5bb6\u5bf9\u98df\u7528\u83cc\u7684\u57f9\u517b\u57fa\u914d\u65b9\u90fd\u975e\u5e38\u719f\u6089\uff0c\u4f46\u662f\u5374\u6ca1\u6709\u5c06\u57f9\u517b\u57fa\u914d\u65b9\u7edf\u4e00\u5f52\u7eb3\u51fa\u6765\uff0c\u5728\u6b64\u8ddf\u5927\u5bb6\u4e00\u8d77\u5206\u4eab\u5e38\u89c1\u7684\u51e0\u79cd\u98df\u7528\u83cc\u6db2\u4f53\u83cc\u79cd\u57f9\u517b\u57fa\u914d\u65b9\u3002 \u3000\u3000
(1) \u5e73\u83c7\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd615\u514b\uff0c\u8461\u8404\u7cd610\u514b\uff0c\u9ea6\u9eb830\u514b\uff0c\u86cb\u767d\u80e81.5\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be1.5\u514b\uff0c\u786b\u9178\u95410.75\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136; \u3000\u3000
(2) \u91d1\u9488\u83c7\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd615\u514b\uff0c\u8461\u8404\u7cd610\u514b\uff0c\u9ea6\u9eb840\u514b\uff0c\u86cb\u767d\u80e82.0\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be2.0\u514b\uff0c\u786b\u9178\u95411.0\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136; \u3000\u3000
(3) \u767d\u7075\u83c7\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd615\u514b\uff0c\u8461\u8404\u7cd610\u514b\uff0c\u9ea6\u9eb840\u514b\uff0c\u86cb\u767d\u80e82.0\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be2.0\u514b\uff0c\u786b\u9178\u95411.0\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136; \u3000\u3000(4)\u9999\u83c7\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd615\u514b\uff0c\u8461\u8404\u7cd610\u514b\uff0c\u9ea6\u9eb840\u514b\uff0c\u86cb\u767d\u80e82.0\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be2.0\u514b\uff0c\u786b\u9178\u95411.0\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136; \u3000\u3000
(4) \u674f\u9c8d\u83c7\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd615\u514b\uff0c\u8461\u8404\u7cd610\u514b\uff0c\u9ea6\u9eb840\u514b\uff0c\u86cb\u767d\u80e82.0\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be2.0\u514b\uff0c\u786b\u9178\u95411.0\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136; \u3000\u3000(6)\u9e21\u817f\u83c7\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd612\u514b\uff0c\u8461\u8404\u7cd612\u514b\uff0c\u9ea6\u9eb840\u514b\uff0c\u86cb\u767d\u80e82.0\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be2.0\u514b\uff0c\u786b\u9178\u95411.0\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136; \u3000\u3000(7)\u9ed1\u6728\u8033\u57f9\u517b\u57fa\u914d\u65b9\u3002\u9a6c\u94c3\u85af100\u514b\uff0c\u7ea2\u7cd615\u514b\uff0c\u8461\u8404\u7cd610\u514b\uff0c\u9ea6\u9eb840\u514b\uff0c\u86cb\u767d\u80e82.0\u514b\uff0c\u78f7\u9178\u4e8c\u6c22\u94be2.0\u514b\uff0c\u786b\u9178\u95411.0\u514b\uff0c\u7ef4\u751f\u7d20B11\u7247\uff0c\u805a\u6c27\u4e19\u7a00\u7518\u6cb90.3\u6beb\u5347\uff0cpH\u503c\u81ea\u7136\u3002
马铃薯葡萄糖琼脂培养基（PDA培养基）：去皮马铃薯200克，葡萄糖20克，琼脂20克，水1000毫升，酸碱度自然。
马铃薯蔗糖琼脂培养基（PSA培养基）：去皮马铃薯200克，蔗糖20克，琼脂20克，水1000毫升，酸碱度自然。
（2）二、三级种培养基配方
木屑麦皮培养基：干杂木屑78%，麦皮20%，蔗糖1%，碳酸钙或熟石膏1%，料∶水=1∶1.2～1.3，酸碱度自然。
菌种培养基配方如下（每升）
胰蛋白胨 10g
牛肉膏粉 5g
氯化钠 2.5g
琼脂 14g
最终pH 6.5±0.1
绛旓細椹搩钖憽钀勭硸鐞艰剛鍩瑰吇鍩猴紙PDA鍩瑰吇鍩猴級锛氬幓鐨┈閾冭柉200鍏嬶紝钁¤悇绯20鍏嬶紝鐞艰剛20鍏嬶紝姘1000姣崌锛岄吀纰卞害鑷劧銆傞┈閾冭柉钄楃硸鐞艰剛鍩瑰吇鍩猴紙PSA鍩瑰吇鍩猴級锛氬幓鐨┈閾冭柉200鍏嬶紝钄楃硸20鍏嬶紝鐞艰剛20鍏嬶紝姘1000姣崌锛岄吀纰卞害鑷劧銆傦紙2锛変簩銆佷笁绾х鍩瑰吇鍩洪厤鏂 鏈ㄥ睉楹︾毊鍩瑰吇鍩猴細骞叉潅鏈ㄥ睉78%锛岄害鐨20%锛岃敆绯1%锛岀⒊閰搁挋鎴栫啛鐭...
绛旓細锛1锛変竴绾х鍩瑰吇鍩洪厤鏂瑰寘鎷┈閾冭柉钁¤悇绯栫惣鑴傚煿鍏诲熀锛圥DA鍩瑰吇鍩猴級锛屽叾閰嶅埗鏂规硶涓猴細鍙栧幓鐨┈閾冭柉200鍏嬶紝鍔犲叆钁¤悇绯20鍏嬪拰鐞艰剛20鍏嬶紝浠1000姣崌姘存憾瑙ｏ紝鑷劧閰哥⒈搴︺傞┈閾冭柉缁煎悎鍩瑰吇鍩猴紙CPDA鍩瑰吇鍩猴級鐨勯厤鏂瑰寘鍚幓鐨┈閾冭柉200鍏嬶紝钁¤悇绯20鍏嬶紝纾烽吀浜屾阿閽3鍏嬶紝纭吀闀1.5鍏嬶紝缁寸敓绱燘10.05姣厠锛岀惣鑴20鍏嬶紝鐢...
绛旓細椹竵姘忓煿鍏诲熀: 鑷劧pH锛屾坊鍔犻摼闇夌礌锛屼笓闂ㄩ拡瀵圭壒瀹氳弻绉嶏紝濡傞┈涓佹皬鍩瑰吇鍩恒椹搩钖煿鍏诲熀: 闇夎弻/閰垫瘝鑿鐨勭悊鎯抽夋嫨锛宲H鑷劧銆侶ayflik鍩瑰吇鍩: 鏋佹瘨锛岄渶鐗瑰埆璋ㄦ厧锛岀敤浜庢瀬灏戞暟鐗规畩瀹為獙銆俁CM鍩瑰吇鍩: 寮哄寲姊弻涓撶敤锛宲H8.5锛屽己鍖栧煿鍏绘鑿岀殑鐢熼暱鏉′欢銆俆YA鍩瑰吇鍩: pH6.5锛岄傚悎鍘屾哀鑿岀殑鐢熼暱锛屽5g鐨勭壒瀹氶厤鏂广傜帀绫抽啰...
绛旓細(1) 骞宠弴鍩瑰吇鍩洪厤鏂广椹搩钖100鍏嬶紝绾㈢硸15鍏嬶紝钁¤悇绯10鍏嬶紝楹﹂焊30鍏嬶紝铔嬬櫧鑳1.5鍏嬶紝纾烽吀浜屾阿閽1.5鍏嬶紝纭吀闀0.75鍏锛岀淮鐢熺礌B11鐗囷紝鑱氭哀涓欑█鐢樻补0.3姣崌锛宲H鍊艰嚜鐒;(2) 閲戦拡鑿囧煿鍏诲熀閰嶆柟銆傞┈閾冭柉100鍏嬶紝绾㈢硸15鍏嬶紝钁¤悇绯10鍏嬶紝楹﹂焊40鍏嬶紝铔嬬櫧鑳2.0鍏嬶紝纾烽吀浜屾阿閽2.0鍏嬶紝纭吀闀1....
绛旓細1銆佺粏鑿屽煿鍏诲熀 閰嶆柟涓 鐗涜倝鑶忕惣鑴傚煿鍏诲熀 鐗涜倝鑶0.3鍏 锛岃泲鐧借儴1.0鍏嬶紝姘寲閽 0.5鍏嬶紝鐞艰剛 1.5鍏嬶紝姘 100姣崌 鍦ㄧ儳鏉唴鍔犳按100姣崌锛屾斁鍏ョ墰鑲夎啅銆佽泲鐧借儴鍜屾隘鍖栭挔锛岀敤铚＄瑪鍦ㄧ儳鏉浣滀笂璁板彿鍚庯紝鏀惧湪鐏笂鍔犵儹銆傚緟鐑ф澂鍐呭悇缁勫垎婧惰В鍚庯紝鍔犲叆鐞艰剛锛屼笉鏂悈鎷屼互鍏嶇矘搴曘傜瓑鐞艰剛瀹屽叏婧惰В鍚庤ˉ瓒冲け姘达紝鐢10...
绛旓細1銆佹瘝绉嶅埗浣滄妧鏈紝涓鑸浜庝繚瀛樻湡鐨勫ぇ鐞冪洊鑿鑿岀鐨勮弻涓濆鍦ㄧ紦鎱㈢敓闀挎垨鍋滄鐢熶骇鐘舵侊紝鎵浠ュ湪鍒朵綔鐢熶骇鐢ㄧ殑姣嶇鍓嶏紝蹇呴』瀵逛繚瀛樼殑鑿岀杩涜澶嶅．锛屼互渚垮皢澶勪簬鏃虹洓鐢熼暱鐘舵佺殑鑿岀鎺ュ叆姣嶇鎴栧師绉嶅煿鍏诲熀涓婏紝淇冭繘鑿屼笣蹇熺敓闀匡紝鑾峰緱鑿屼笣绮楀．銆佹祿鐧姐佺敓闀垮娍鏃虹殑鑿岀銆傦紙1锛鍩瑰吇鍩洪厤鏂锛欼鍙凤細PDA(椹搩钖200g銆佽憽钀勭硸...
绛旓細榛戞湪鑰充簩绾х锛堝師绉嶏級銆佷笁绾х锛堟牻鍩圭锛夊埗浣滀笌榛戞湪鑰崇浉浼硷紝閫氬父鏄噰鐢ㄦ潅鏈ㄥ睉锛堟垨妫夌苯澹虫垨鏉傛湪鏋濇潯锛夐焊鐨鍩瑰吇鍩锛屼簩绾х浠750姣崌鑿岀鐡朵负瀹瑰櫒銆備笁绾х浠ヨ弻鐡舵垨鑿岃涓哄鍣ㄣ備负纭繚鑿岀鐢熼暱鍙戣偛鑹ソ锛屽湪鍩瑰吇鍩洪夋枡鍜閰嶅埗涓婂繀椤绘妸鎻″ソ浠ヤ笅鍚勭偣锛氣憼鎷屾枡銆傛妸骞叉湪灞戯紝鍚岄害楹告垨绫崇碃銆佺煶鑶忕矇鎴栫⒊閰搁挋鍏堟悈鎷屽潎鍖...
绛旓細鍘熺鍜屾牻鍩圭鍩瑰吇鍩虹殑閰嶆柟鍩鏈浉鍚岋紝鍒朵綔鏂规硶涔熺浉浼笺備负閬垮厤閲嶅锛屼笅闈㈢畝浠嬪嚑绉嶅父瑙佺殑鍩瑰吇鍩洪厤鏂鍙婂埗娉曘傦紙1锛夋湪灞戝煿鍏诲熀閫傚疁浜庣伒鑺鑿岀 闃斿彾鏍戞湪灞78%锛岃敆绯1%锛岄焊鐨紙鎴栫背绯狅級20%锛岀煶鑶忕矇1%锛堟垨纰抽吀閽1%锛夈傛垨鍔犱竴瀹氭瘮渚嬬殑灏跨礌銆佺⒊閰搁挋銆佺７閰镐簩姘㈤捑銆傞様鍙舵爲鏈ㄥ睉78%锛岀帀绫崇矇20%锛岀煶鑶忕矇1%锛屽翱绱1%...
绛旓細鍏舵瘝绉鍩瑰吇鍩锛屽父鐢ㄤ互涓嬩袱绉閰嶆柟锛氶厤鏂逛竴锛氶┈閾冭柉200鍏嬶紝鐞艰剛20鍏嬶紝钁¤悇绯20鍏嬶紝鍚爱鍖栧悎鐗╅傞噺锛屾按1000姣崌銆傞厤鏂逛簩锛氶┈閾冭柉200鍏嬶紝鐞艰剛20鍏嬶紝钁¤悇绯20鍏嬶紝鍚敓闀垮洜绱犵殑鐗╄川灏戦噺锛岀７閰镐簩姘㈤捑3鍏嬶紝纭吀闀1.5鍏嬶紝姘1000姣崌銆傚埗浣滄椂锛屽厛灏嗗湡璞嗗幓鐨垏鐗囧悗缃鍣ㄥ唴锛屽姞姘1000姣崌锛屽姞鐑叜娌30鍒嗛挓锛岀叜鑷...
绛旓細铇戣弴鐨勪竴绾х涔熷父鐢≒DA鏂滈潰璇曠绉嶏紝鍗楁柟鍚勫湴鍙堢О姣嶇銆傚叾閰嶆柟鏄幓鐨┈閾冭柉200鍏嬶紝钁¤悇绯20鍏嬶紝鐞艰剛20鍏嬶紝姘1000姣崌锛宲H鑷劧銆傝槕鑿囦竴绾х鍩瑰吇鍩洪厤鏂鐣ユ湁涓嶅悓锛屼絾鐢熶骇宸ヨ壓鍜屽埗浣滄柟娉曞熀鏈浉鍚屻備互椹搩钖煿鍏诲熀鍒朵綔鏂规硶浠嬬粛濡備笅锛氣憼灏嗛┈閾冭柉娲楀噣鍘荤毊锛堝凡鍙戣娊鐨勮鎸栨帀鑺界溂锛夛紝绉伴噺鍙200鍏嬪垏鎴愯杽鐗囷紝缃簬閾濋攨...
